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Literature summary extracted from

  • Yamada, J.; Kurata, A.; Hirata, M.; Taniguchi, T.; Takama, H.; Furihata, T.; Shiratori, K.; Iida, N.; Takagi-Sakuma, M.; Watanabe, T.; Kurosaki, K.; Endo, T.; Suga, T.
    Purification, molecular cloning, and genomic organization of human brain long-chain acyl-CoA hydrolase (1999), J. Biochem., 126, 1013-1019.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 DNA sequence determination and analysis, chromosome mapping 1p36.2, expression in Escherichia coli Homo sapiens
3.1.2.20 DNA sequence determination and analysis, chromosome mapping 1p36.2, expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 p-chloromercuribenzoic acid
-
 Homo sapiens
3.1.2.20 p-chloromercuribenzoic acid
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.0064
-
 hexadecanoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.2 0.0076
-
 dodecanoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.2 0.0088
-
 oleoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.2 0.0092
-
 Arachidonoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.2 0.014
-
 octanoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.20 0.0064
-
 hexadecanoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.20 0.0076
-
 dodecanoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.20 0.0088
-
 oleoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.20 0.0092
-
 Arachidonoyl-CoA pH 7.4, 30°C Homo sapiens
3.1.2.20 0.014
-
 octanoyl-CoA pH 7.4, 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Homo sapiens 5829
-
3.1.2.20 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 43000
-
 n * 43000, enzyme is a dimer or a trimer of MW 100 kDa, SDS-PAGE Homo sapiens
3.1.2.2 100000
-
 gel filtration Homo sapiens
3.1.2.20 43000
-
 n * 43000, enzyme is a dimer or a trimer of MW 100 kDa, SDS-PAGE Homo sapiens
3.1.2.20 100000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Homo sapiens O00154 human brain long-chain acyl-CoA hydrolase hBACH
-
3.1.2.20 Homo sapiens O00154 human brain long-chain acyl-CoA hydrolase hBACH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 from brain Homo sapiens
3.1.2.20 from brain Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 brain
-
 Homo sapiens
-
3.1.2.20 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 198
-
 purified native enzyme, substrate palmitoyl-CoA Homo sapiens
3.1.2.20 198
-
 purified native enzyme, substrate palmitoyl-CoA Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O acyl-CoA of chain length C8-C18 Homo sapiens CoA + a carboxylate
-
 ?
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Homo sapiens CoA + a carboxylate
-
 ?
3.1.2.2 arachidonoyl-CoA + H2O
-
 Homo sapiens CoA + arachidonate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O
-
 Homo sapiens CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O best substrate Homo sapiens CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Homo sapiens CoA + hexadecanoate
-
 ?
3.1.2.2 octanoyl-CoA + H2O
-
 Homo sapiens CoA + octanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Homo sapiens CoA + oleate
-
 ?
3.1.2.20 acyl-CoA + H2O acyl-CoA of chain length C8-C18 Homo sapiens CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Homo sapiens CoA + a carboxylate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O
-
 Homo sapiens CoA + arachidonate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O
-
 Homo sapiens CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O best substrate Homo sapiens CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Homo sapiens CoA + hexadecanoate
-
 ?
3.1.2.20 octanoyl-CoA + H2O
-
 Homo sapiens CoA + octanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Homo sapiens CoA + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 oligomer n * 43000, enzyme is a dimer or a trimer of MW 100 kDa, SDS-PAGE Homo sapiens
3.1.2.20 oligomer n * 43000, enzyme is a dimer or a trimer of MW 100 kDa, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 30
-
 assay at Homo sapiens
3.1.2.20 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7.4
-
 assay at Homo sapiens
3.1.2.20 7.4
-
 assay at Homo sapiens