EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.6.17 | 2-Chloropropionate | 20 mM, 8% inhibition | Pseudomonas syringae | |
3.1.6.17 | butan-2-yl-sulfate | 20 mM, 25% inhibition | Pseudomonas syringae | |
3.1.6.17 | butyrate-2-sulfate | 20 mM, 42% inhibition | Pseudomonas syringae | |
3.1.6.17 | D-lactate | product inhibition, kinetics | Pseudomonas syringae | |
3.1.6.17 | D-serine-O-sulfate | 20 mM, 11% inhibition | Pseudomonas syringae | |
3.1.6.17 | glycolate-2-sulfate | 20 mM, 43% inhibition | Pseudomonas syringae | |
3.1.6.17 | Hexan-2-yl sulfate | 20 mM, 13% inhibition | Pseudomonas syringae | |
3.1.6.17 | L-lactate 2-sulfate | 12 mM, 43% inhibition | Pseudomonas syringae | |
3.1.6.17 | pentan-3-yl hydrogen sulfate | 20 mM, 10% inhibition | Pseudomonas syringae | |
3.1.6.17 | propan-1-yl sulfate | 20 mM, 22% inhibition | Pseudomonas syringae | |
3.1.6.17 | propan-2-yl-sulfate | 20 mM, 18% inhibition | Pseudomonas syringae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.6.17 | 0.0067 | - |
D-Lactate 2-sulfate | pH 7.5, 25°C | Pseudomonas syringae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.6.17 | soluble | - |
Pseudomonas syringae | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.6.17 | 55000 | - |
1 * 55000, Pseudomonas syringae, SDS-PAGE | Pseudomonas syringae |
3.1.6.17 | 59800 | - |
PAGE, gel filtration yields a higher MW, presumably due to aggregation | Pseudomonas syringae |
3.1.6.17 | 89000 | - |
gel filtration, high MW presumably due to aggregation | Pseudomonas syringae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.6.17 | Pseudomonas syringae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.6.17 | - |
Pseudomonas syringae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.6.17 | 10.9 | - |
pH 7.5, 25°C | Pseudomonas syringae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.6.17 | 4-nitrophenyl sulfate + H2O | at 6% the rate of D-lactate 2-sulfate hydrolysis | Pseudomonas syringae | 4-nitrophenol + SO42- | - |
? | |
3.1.6.17 | D-lactate 2-sulfate + H2O | stereospecific, not L-lactate 2-sulfate, cleaves O-S-bond of the C-O-S-ester link | Pseudomonas syringae | D-lactate + SO42- | not L-lactate | ? | |
3.1.6.17 | additional information | no substrates are the sulfate esters of L-lactate, methanol, ethanol, propanol, propan-2-ol, butanol, butan-2-ol, pentanol, pentan-2-ol, pentan-3-ol, hexanol, hexan-2-ol, heptanol, heptan-2-ol, heptan-4-ol, octanol, octan-2-ol, decanol, decan-5-ol, 2-hydroxybutyrate, 2-hydroxyisobutyrate, butoxyethanol and phenoxyethanol, the 6-sulfate esters of glucosamine and N-acetylglucosamine and the 3-sulfate esters of glucosamine and N-acetylgalactosamine. No dehalogenase activity: potassium DL-chloropropionate is not a substrate | Pseudomonas syringae | ? | - |
? | |
3.1.6.17 | serine-O-sulfate + H2O | at 9% the rate of D-lactate 2-sulfate hydrolysis | Pseudomonas syringae | serine + SO42- | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.6.17 | monomer | 1 * 55000, Pseudomonas syringae, SDS-PAGE | Pseudomonas syringae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.6.17 | 35 | - |
- |
Pseudomonas syringae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.6.17 | 25 | - |
pH 7.5, stable up to, 5 min | Pseudomonas syringae |
3.1.6.17 | 50 | - |
pH 7.5, 5 min, inactivation | Pseudomonas syringae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.6.17 | 7.5 | - |
0.2 M Tris-HCl buffer | Pseudomonas syringae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.6.17 | 16.3 | - |
L-lactate-2-sulfate | pH 7.5, 25°C | Pseudomonas syringae |