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Literature summary extracted from

  • Kobayashi, T.; Kanai, H.; Hayashi, T.; Akiba, T.; Akaboshi, R.; Horikoshi, K.
    Haloalkaliphilic maltotriose-forming alpha-amylase from the archaebacterium Natronococcus sp. strain Ah-36 (1992), J. Bacteriol., 174, 3439-3444.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.1.116 0.5% soluble starch stabilizes Natronococcus sp.
3.2.1.116 2.5 M NaCl stabilizes Natronococcus sp.
3.2.1.116 activity is irreversibly lost at low ionic strength Natronococcus sp.
3.2.1.116 no loss of activity after dialysis Natronococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.116 alpha-cyclodextrin competitive inhibitor, 0.25% Natronococcus sp.
3.2.1.116 beta-cyclodextrin competitive inhibitor, 0.025% Natronococcus sp.
3.2.1.116 Ca2+ 1 mM, 33% inhibition Natronococcus sp.
3.2.1.116 Hg2+ weak, 1 mM: 15% inhibition, 10 mM: 75% inhibition Natronococcus sp.
3.2.1.116 additional information not inhibited by 1 mM iodoacetic acid, 1 mM phenylmethylsulfonyl fluoride Natronococcus sp.
3.2.1.116 N-bromosuccinimide 1 mM, 73% inhibition Natronococcus sp.
3.2.1.116 Ni2+ 1 mM, 33% inhibition Natronococcus sp.
3.2.1.116 SDS 0.1%, 80% inhibition Natronococcus sp.
3.2.1.116 Urea 1 M, 74% inhibition Natronococcus sp.
3.2.1.116 ZnCl2 1 mM, complete inhibition Natronococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.116 6.8
-
gamma-cyclodextrin pH 9, 50°C, Km-value shown as glucose equivalents Natronococcus sp.
3.2.1.116 19
-
amylose pH 9, 50°C, DP, 17, Km-value shown as glucose equivalents Natronococcus sp.
3.2.1.116 25
-
starch pH 9, 50°C, soluble, Km-value shown as glucose equivalents Natronococcus sp.
3.2.1.116 42
-
glycogen pH 9, 50°C, from oyster, Km-value shown as glucose equivalents Natronococcus sp.
3.2.1.116 49
-
amylopectin pH 9, 50°C, Km-value shown as glucose equivalents Natronococcus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.116 extracellular
-
Natronococcus sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.116 CsCl can partially substitute for NaCl at higher concentrations Natronococcus sp.
3.2.1.116 KCl can partially substitute for NaCl at higher concentrations Natronococcus sp.
3.2.1.116 NaCl requirement, maximum activity at 2.5 M NaCl, no activity below 1 M NaCl Natronococcus sp.
3.2.1.116 RbCl can partially substitute for NaCl at higher concentrations Natronococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.116 74000
-
1 * 74000, SDS-PAGE Natronococcus sp.
3.2.1.116 76000
-
gel filtration Natronococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.116 Natronococcus sp.
-
-
-
3.2.1.116 Natronococcus sp. Ah-36
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.116 2000fold, in the presence of 2.5 M NaCl Natronococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.116 160
-
pH 9, 50°C Natronococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.116 3 glycogen + H2O from oyster, slow hydrolysis Natronococcus sp. 4 maltotriose additional small amounts of maltose and glucose, alpha-configuration ?
3.2.1.116 amylopectin + H2O
-
Natronococcus sp. maltotriose additional small amounts of maltose and glucose, alpha-configuration ?
3.2.1.116 amylose + H2O
-
Natronococcus sp. maltotriose
-
?
3.2.1.116 gamma-cyclodextrin + H2O
-
Natronococcus sp. ?
-
?
3.2.1.116 maltoheptaose + H2O
-
Natronococcus sp. maltotriose main product, additional small amounts of maltose and glucose, alpha-configuration ?
3.2.1.116 maltohexaose + H2O
-
Natronococcus sp. maltotriose + ? alpha-configuration ?
3.2.1.116 maltohexaose + H2O
-
Natronococcus sp. maltotriose + ? main product, additional small amounts of maltose and glucose ?
3.2.1.116 maltohexaose + H2O
-
Natronococcus sp. Ah-36 maltotriose + ? alpha-configuration ?
3.2.1.116 maltohexaose + H2O
-
Natronococcus sp. Ah-36 maltotriose + ? main product, additional small amounts of maltose and glucose ?
3.2.1.116 maltooligosaccharide + H2O ranging from maltotetraose to maltoheptaose, not: maltotriose, maltose Natronococcus sp. maltotriose alpha-configuration ?
3.2.1.116 maltopentaose + H2O
-
Natronococcus sp. maltotriose + D-maltose alpha-configuration ?
3.2.1.116 maltopentaose + H2O
-
Natronococcus sp. maltotriose + D-maltose additional small amounts of glucose ?
3.2.1.116 maltotetraose + H2O
-
Natronococcus sp. maltotriose + D-glucose alpha-configuration, additional product: D-maltose ?
3.2.1.116 additional information not: alpha-cyclodextrin, pullulan Natronococcus sp. ?
-
?
3.2.1.116 additional information not: maltotriose, maltose, beta-cyclodextrin Natronococcus sp. ?
-
?
3.2.1.116 additional information enzyme has transferase activity using maltopentaose or maltotetraose as substrates Natronococcus sp. ?
-
?
3.2.1.116 additional information not: alpha-cyclodextrin, pullulan Natronococcus sp. Ah-36 ?
-
?
3.2.1.116 additional information not: maltotriose, maltose, beta-cyclodextrin Natronococcus sp. Ah-36 ?
-
?
3.2.1.116 additional information enzyme has transferase activity using maltopentaose or maltotetraose as substrates Natronococcus sp. Ah-36 ?
-
?
3.2.1.116 short-chain amylose + H2O DP, 17, most rapid hydrolysis Natronococcus sp. maltotriose alpha-configuration ?
3.2.1.116 short-chain amylose + H2O DP, 17, most rapid hydrolysis Natronococcus sp. maltotriose additional small amounts of maltose and glucose ?
3.2.1.116 starch + H2O soluble Natronococcus sp. maltotriose + ? additional small amounts of maltose and glucose, alpha-configuration ?

Subunits

EC Number Subunits Comment Organism
3.2.1.116 monomer 1 * 74000, SDS-PAGE Natronococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.116 50
-
assay at Natronococcus sp.
3.2.1.116 55
-
pH 9, 30 min, in the presence of 2.5 M NaCl Natronococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.116 additional information
-
soluble starch stabilizes against thermal inactivation, Ca2+ does not enhance thermal stability Natronococcus sp.
3.2.1.116 50
-
30 min, 2.5 M NaCl, pH 4.2: 80% loss of activity, pH 5.0: 44% loss of activity, pH 6.0-8.6: stable, pH 10.5: 30% loss of activity Natronococcus sp.
3.2.1.116 55
-
pH 8.0, 30 min, 2.5 M NaCl, 50% loss of activity, no loss of activity in the presence of 0.5% soluble starch Natronococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.116 8.7
-
in the presence of 2.5 M NaCl Natronococcus sp.
3.2.1.116 9
-
assay at Natronococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.116 3.5
-
30 min, 50°C, 2.5 M NaCl, 0.5% soluble starch, 80% loss of activity Natronococcus sp.
3.2.1.116 4.2
-
30 min, 50°C, 2.5 M NaCl, 80% loss of activity Natronococcus sp.
3.2.1.116 5
-
30 min, 50°C, 2.5 M NaCl, 44% loss of activity Natronococcus sp.
3.2.1.116 6 10 30 min, 50°C, 2.5 M NaCl, 0.5% soluble starch, stable Natronococcus sp.
3.2.1.116 6 8.6 30 min, 50°C, 2.5 M NaCl, stable Natronococcus sp.
3.2.1.116 10.5
-
30 min, 50°C, 2.5 M NaCl, 30% loss of activity Natronococcus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.116 0.41
-
beta-cyclodextrin pH 9, 50°C, 0.025% Natronococcus sp.
3.2.1.116 1.63
-
alpha-cyclodextrin pH 9, 50°C, 0.25% Natronococcus sp.