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Literature summary extracted from

  • Shigeoka, S.; Nakano, Y.
    Characterization and molecular properties of 2-oxoglutarate decarboxylase from Euglena gracilis (1991), Arch. Biochem. Biophys., 288, 22-28.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.71 2-mercaptoethanol stimulates Euglena gracilis
4.1.1.71 DTT stimulates Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.71 Cu2+
-
 Euglena gracilis
4.1.1.71 Hg2+
-
 Euglena gracilis
4.1.1.71 additional information not: NaN3 Euglena gracilis
4.1.1.71 NEM
-
 Euglena gracilis
4.1.1.71 Ni2+
-
 Euglena gracilis
4.1.1.71 PCMB
-
 Euglena gracilis
4.1.1.71 Succinic semialdehyde
-
 Euglena gracilis
4.1.1.71 Zn2+
-
 Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.71 0.33
-
 2-oxoglutarate pH 7.0 Euglena gracilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.71 Ca2+ no effect Euglena gracilis
4.1.1.71 Co2+ no effect Euglena gracilis
4.1.1.71 MgCl2 stimulates Euglena gracilis
4.1.1.71 MgCl2 Km: 0.093 mM Euglena gracilis
4.1.1.71 Mn2+ stimulates Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.71 62000
-
 4 * 62000, SDS-PAGE Euglena gracilis
4.1.1.71 250000
-
 gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.71 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.71
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.71 2-oxoglutarate highly specific Euglena gracilis succinate semialdehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.71 tetramer 4 * 62000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.71 42.5
-
-
 Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.71 57.5
-
 10 min, stable up to Euglena gracilis
4.1.1.71 62
-
 pH 6.2-8.5, 10 min, stable Euglena gracilis
4.1.1.71 67
-
 10 min, complete loss of activity Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.71 7
-
-
 Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.71 6 9 pH 6.0: 65% of activity maximum, pH 9.0: 55% of activity maximum Euglena gracilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.71 6.2 8.5 62°C, 10 min, stable Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.71 ATP slight stimulation Euglena gracilis
4.1.1.71 NADP+ stimulates Euglena gracilis
4.1.1.71 NADPH stimulates Euglena gracilis
4.1.1.71 thiamine diphosphate required Euglena gracilis
4.1.1.71 thiamine diphosphate Km: 0.056 mM Euglena gracilis