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Literature summary extracted from

  • Schneider-Belhaddad, F.; Kolattukudy, P.
    Solubilization, partial purification, and characterization of a fatty aldehyde decarbonylase from a higher plant, Pisum sativum (2000), Arch. Biochem. Biophys., 377, 341-349.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.99.5 phopshatidylserine requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively Pisum sativum
4.1.99.5 phosphatidylcholine requirement, preferred lipid, phopshatidylserine and phopshatidylethanolamine substitute less effectively Pisum sativum
4.1.99.5 phosphatidylethanolamine requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively Pisum sativum
4.1.99.5 Phospholipid requirement, dose-dependent stimulation, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.5 1,10-phenanthroline 1 mM, complete inhibition, CuCl2 restores, CoCl2 and ZnCl2 partially restore activity; strong Pisum sativum
4.1.99.5 dithioerythritol 5 mM, complete inhibition Pisum sativum
4.1.99.5 EDTA 10 mM, 58% inhibition Pisum sativum
4.1.99.5 metal ion chelators Cu2+ fully restores activity; strong Pisum sativum
4.1.99.5 NADH presence causes 50% inhibition Pisum sativum
4.1.99.5 NADPH presence causes 50% inhibition Pisum sativum
4.1.99.5 O2 complete inhibition Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.99.5 membrane membrane-associated Pisum sativum 16020
-
4.1.99.5 microsome membrane-associated Pisum sativum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.5 Cu2+ probably a copper enzyme Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.5 67000
-
 x * 67000, SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.5 additional information Pisum sativum alkane biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.5 Pisum sativum
-
 L. V Early Frosty
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.99.5 inhibited in the presence of O2 Pisum sativum

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.5 partial, 1306fold, solubilized with beta-octyl glucoside Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.99.5 leaf young rapidly growing Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.5 0.000235
-
 at room temperature Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.5 additional information alkane biosynthesis Pisum sativum ?
-
 ?
4.1.99.5 octadecanal
-
 Pisum sativum heptadecane + CO
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.5 ? x * 67000, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.5 additional information
-
 assay at room temperature Pisum sativum