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Literature summary extracted from

  • Sachulova, J.; Satava, J.; Veleminsky, J.
    A barley endonuclease specific for apurinic DNA. Isolation and partial characterization (1978), Eur. J. Biochem., 87, 215-220.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.99.18 16000
-
 gel filtration Hordeum vulgare
4.2.99.18 20000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.99.18 DNA Hordeum vulgare
-
 fragments of DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.99.18 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.99.18 partial Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.99.18 leaf
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.99.18 additional information
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.99.18 DNA
-
 Hordeum vulgare fragments of DNA
-
 ?
4.2.99.18 DNA at low concentrations the enzyme is specific for depurinated native DNA, at higher concentrations it degrades DNA in a non-specific manner Hordeum vulgare fragments of DNA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.99.18 monomer 1 * 20000, SDS-PAGE Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.99.18 7.5
-
-
 Hordeum vulgare
4.2.99.18 7.6
-
-
 Hordeum vulgare