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Literature summary extracted from

  • Kogekar, R.G.; Desphpande, V.N.
    Biosynthesis of Penicillin in vitro: purification & properties of phenyl/phenoxyacetic acid activating enzyme (1982), Indian J. Biochem. Biophys., 19, 257-261.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.30 2-mercaptoethanol 0.01 mM, 100% stimulation Penicillium chrysogenum
6.2.1.30 dithiothreitol 0.01 mM, 80% stimulation Penicillium chrysogenum
6.2.1.30 GSH 0.01 mM, 60% stimulation Penicillium chrysogenum

General Stability

EC Number General Stability Organism
6.2.1.30 2-mercaptoethanol stabilizes Penicillium chrysogenum
6.2.1.30 repeated freezing and thawing inactivates Penicillium chrysogenum
6.2.1.30 sucrose stabilizes Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.30 Fe2+ 0.01 mM, complete inhibition Penicillium chrysogenum
6.2.1.30 NEM 0.01 mM, complete inhibition Penicillium chrysogenum
6.2.1.30 p-chloromercuribenzoate 0.01 mM, complete inhibition Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.30 0.0029
-
 phenylacetate pH 6.8, 37°C Penicillium chrysogenum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.30 Mg2+ 0.01 mM, required, cannot be substituted by Mn2+, Zn2+, Na+, K+ Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.30 Penicillium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.30
-
 Penicillium chrysogenum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.30 mycelium
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.30 0.199
-
-
 Penicillium chrysogenum

Storage Stability

EC Number Storage Stability Organism
6.2.1.30 0°C, 24 h, very little activity retained, sucrose and 2-mercaptoethanol stabilize Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.30 ATP + phenoxyacetic acid + CoA
-
 Penicillium chrysogenum AMP + phenoxyacetyl-CoA + diphosphate
-
 ?
6.2.1.30 ATP + phenoxyacetic acid + hydroxylamine
-
 Penicillium chrysogenum AMP + phenylacetoxyhydroxamate + diphosphate
-
 ?
6.2.1.30 ATP + phenylacetate + CoA
-
 Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetic acid + hydroxylamine
-
 Penicillium chrysogenum AMP + phenylacetylhydroxamate + diphosphate
-
 ?
6.2.1.30 additional information only beta-substitued acetic acid can serve as substrate, not phenylpropionic acid Penicillium chrysogenum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.30 37
-
-
 Penicillium chrysogenum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.30 32 42 32°C: about 40% of maximal activity, 42°C: about 40% of maximal activity Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.30 6.8
-
-
 Penicillium chrysogenum