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Literature summary extracted from

  • Farkas, A.; Tompa, P.; Schad, E.; Sinka, R.; Jekely, G.; Friedrich, P.
    Autolytic activation and localization in Schneider cells (S2) of calpain B from Drosophila (2003), Biochem. J., 378, 299-305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B37 mutant enzyme Q73G/N74V/A75P/N223A/Q224V expressed in Escherichia coli Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.B37 Q73G/N74V/A75P/N223A/Q224V mutation within the autolytic cleavage sites, autolysis is not arrested by the mutations but its site shifts to new, nearby peptide bonds. In the case of site between Q224 and N225, two new sites emerge: one at F215-T216 and one at G230-R231. In the case of site between N74 and A75, modification gives rise to low intensity, blurred bands which can not be analyzed by sequencing Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B37 membrane
-
 Drosophila melanogaster 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.B37 Ca2+ stimulation with Ca2+ results in autolytic conversion from the 104000 Da to a 81000 Da form with intermediary fragments. The two major products of autolysis appear at 96000 Da and 81000 Da Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B37 104000
-
 x * 104000, SDS-PAGE, calculation from nucleotide sequence Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B37 Drosophila melanogaster
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.B37 egg high activity Drosophila melanogaster
-
3.4.22.B37 embryo in the late embryos high activity is detected in the tracheas and their orifices as well as in the larynx Drosophila melanogaster
-
3.4.22.B37 imago
-
 Drosophila melanogaster
-
3.4.22.B37 larva in the early larval stage the calpain B level is very low. After this stage the level rises strongly, indicating the vigorous transcription of the CalpB gene up to the third larval stage, then the level becomes constant in the pupa and imago Drosophila melanogaster
-
3.4.22.B37 larynx in the late embryos high activity is detected in the tracheas and their orifices as well as in the larynx Drosophila melanogaster
-
3.4.22.B37 additional information calpain B is detectable in all developmental stages of the fly Drosophila melanogaster
-
3.4.22.B37 oocyte
-
 Drosophila melanogaster
-
3.4.22.B37 ovarian follicle activity is strongly detectable in follicular and border cells of the oocyte Drosophila melanogaster
-
3.4.22.B37 ovary
-
 Drosophila melanogaster
-
3.4.22.B37 pupa
-
 Drosophila melanogaster
-
3.4.22.B37 salivary gland high expression Drosophila melanogaster
-
3.4.22.B37 Schneider S2 cell
-
 Drosophila melanogaster
-
3.4.22.B37 trachea in the late embryos high activity is detected in the tracheas and their orifices as well as in the larynx Drosophila melanogaster
-

Subunits

EC Number Subunits Comment Organism
3.4.22.B37 ? x * 104000, SDS-PAGE, calculation from nucleotide sequence Drosophila melanogaster