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Literature summary extracted from

  • Filipowicz, W.; Billy, E.; Drabikowski, K.; Genschik, P.
    Cyclases of the 3'-terminal phosphate in RNA: a new family of RNA processing enzymes conserved in Eucarya, Bacteria and Archaea (1998), Acta Biochim. Pol., 45, 895-906.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.4 cloned and overexpressed in Escherichia coli as fusion protein with glutathione S-transferase Homo sapiens
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Drosophila melanogaster
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Mus musculus
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Saccharomyces cerevisiae
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Arabidopsis thaliana
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Dictyostelium discoideum
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Schizosaccharomyces pombe
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Caenorhabditis elegans
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Danio rerio
6.5.1.4 expressed sequence tags encoding cyclase like protein identified Toxoplasma gondii
6.5.1.4 gene encoding cyclase like protein present Pseudomonas aeruginosa
6.5.1.4 gene encoding cyclase like protein present Methanocaldococcus jannaschii
6.5.1.4 gene encoding cyclase like protein present Aquifex aeolicus
6.5.1.4 gene rtcA encoding cyclase cloned and overexpressed Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.4 NaCl
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.4 0.006
-
 ATP
-
 Homo sapiens
6.5.1.4 0.02
-
 ATP
-
 Escherichia coli
6.5.1.4 0.1
-
 GTP
-
 Escherichia coli
6.5.1.4 0.2
-
 GTP
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.5.1.4 nucleus
-
 Xenopus sp. 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.4 Mg2+
-
 Escherichia coli
6.5.1.4 Mn2+ able to replace Mg2+ Escherichia coli
6.5.1.4 additional information no activity with Ca2+, Zn2+, or Cu2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.4 39400
-
 calculated from cDNA Homo sapiens
6.5.1.4 40000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.4 Aquifex aeolicus
-
-
-
6.5.1.4 Arabidopsis thaliana
-
-
-
6.5.1.4 Caenorhabditis elegans
-
-
-
6.5.1.4 Danio rerio
-
 zebra fish
-
6.5.1.4 Dictyostelium discoideum
-
-
-
6.5.1.4 Drosophila melanogaster
-
 fruit fly
-
6.5.1.4 Escherichia coli
-
-
-
6.5.1.4 Homo sapiens
-
 human
-
6.5.1.4 Methanocaldococcus jannaschii
-
-
-
6.5.1.4 Mus musculus
-
 mouse
-
6.5.1.4 no activity in Bacillus subtilis
-
-
-
6.5.1.4 no activity in Haemophilus influenzae
-
-
-
6.5.1.4 no activity in Helicobacter pylori
-
-
-
6.5.1.4 no activity in Mycoplasma genitalium
-
-
-
6.5.1.4 no activity in Synechocystis sp.
-
-
-
6.5.1.4 Pseudomonas aeruginosa
-
-
-
6.5.1.4 Saccharomyces cerevisiae
-
 yeast
-
6.5.1.4 Schizosaccharomyces pombe
-
 fission yeast
-
6.5.1.4 Toxoplasma gondii
-
-
-
6.5.1.4 Xenopus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.4
-
 Escherichia coli
6.5.1.4 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.4 ATP + (Ap)npAp pure oligoribonucleotide Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + (Up)10pGp pure oligoribonucleotide Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + 5S rRNA modified by ligation of [5'-P]pCp to the 3'-terminus Escherichia coli AMP + ?
-
 ?
6.5.1.4 ATP + 5S rRNA-pCp
-
 Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + AAAAUAAAAGCp pure oligoribonucleotide Escherichia coli AMP + ?
-
 ?
6.5.1.4 ATP + AAAAUAAAAGCp pure oligoribonucleotide Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + AAAAUAAAAGp pure oligoribonucleotide Escherichia coli AMP + ?
-
 ?
6.5.1.4 ATP + AAAAUAAAAGp pure oligoribonucleotide Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + AUGp pure oligoribonucleotide Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + CCCCACCCCGp pure oligoribonucleotide Escherichia coli AMP + ?
-
 ?
6.5.1.4 ATP + CCCCACCCCGp pure oligoribonucleotide Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + CCCCCACCCCGCp pure oligoribonucleotide Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + human U14 snoRNA modified by ligation of [5'-P]pCp to the 3'-terminus Escherichia coli AMP + ?
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Drosophila melanogaster AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Mus musculus AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Escherichia coli AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Saccharomyces cerevisiae AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Arabidopsis thaliana AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Pseudomonas aeruginosa AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Dictyostelium discoideum AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Schizosaccharomyces pombe AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Caenorhabditis elegans AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Xenopus sp. AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Methanocaldococcus jannaschii AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Danio rerio AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Aquifex aeolicus AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate
-
 Toxoplasma gondii AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + tobacco mosaic virus RNA fragments modified by ligation of pGp, pAp, or pCp Homo sapiens AMP + ?
-
 ?
6.5.1.4 ATP + tRNA-pNp
-
 Homo sapiens AMP + ?
-
 ?
6.5.1.4 GTP + RNA 3'-terminal-phosphate
-
 Escherichia coli GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 GTP + RNA 3'-terminal-phosphate
-
 Homo sapiens GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 additional information nucleoside 3'-monophosphates and nucleoside 5',3'-bisphosphates are no substrates Escherichia coli ?
-
 ?
6.5.1.4 additional information trinucleotides are the shortest oligonucleotides able to act as substrates, ribonucleoside 3'-phosphates and ribonucleoside 5',3'-phosphates are no substrates Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.4 RNA 3'-teminal phosphate cyclase
-
 Methanocaldococcus jannaschii
6.5.1.4 RNA 3'-teminal phosphate cyclase
-
 Aquifex aeolicus
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Drosophila melanogaster
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Mus musculus
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Escherichia coli
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Homo sapiens
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Saccharomyces cerevisiae
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Arabidopsis thaliana
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Pseudomonas aeruginosa
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Dictyostelium discoideum
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Schizosaccharomyces pombe
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Caenorhabditis elegans
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Xenopus sp.
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Danio rerio
6.5.1.4 RNA 3'-terminal phosphate cyclase
-
 Toxoplasma gondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.4 8 9
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.4 ATP
-
 Escherichia coli
6.5.1.4 ATP requires ATP as cofactor, other ribonucleoside triphosphates are used very inefficiently Homo sapiens