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Literature summary extracted from

  • Tsuge, T.; Hisano, T.; Taguchi, S.; Doi, Y.
    Alteration of chain length substrate specificity of Aeromonas caviae R-enantiomer-specific enoyl-coenzyme A hydratase through site-directed mutagenesis (2003), Appl. Environ. Microbiol., 69, 4830-4836.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.119
-
 Aeromonas caviae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.119 L654G/V130G decreased specific activity for crotonyl-CoA Aeromonas caviae
4.2.1.119 L65A enzyme activity similar to wild-type enzyme Aeromonas caviae
4.2.1.119 L65A/V130G decreased specific activity for crotonyl-CoA Aeromonas caviae
4.2.1.119 L65G enzyme activity similar to wild-type enzyme Aeromonas caviae
4.2.1.119 L65I specific activity with crotonyl-CoA similar to wild-type enzyme Aeromonas caviae
4.2.1.119 L65V specific activity with crotonyl-CoA similar to wild-type enzyme Aeromonas caviae
4.2.1.119 S62A decreased specific activity for crotonyl-CoA Aeromonas caviae
4.2.1.119 V130A specific activity with crotonyl-CoA similar to wild-type enzyme Aeromonas caviae
4.2.1.119 V130G enzyme activity similar to wild-type enzyme, lower structural stability than wild-type enzyme Aeromonas caviae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.119 0.005
-
 dodec-2-enoyl-CoA pH 8, 30°C, V130G mutant Aeromonas caviae
4.2.1.119 0.013
-
 dec-2-enoyl-CoA pH 8, 30°C, V130G mutant Aeromonas caviae
4.2.1.119 0.018
-
 hexenoyl-CoA pH 8, 30°C, L65A mutant Aeromonas caviae
4.2.1.119 0.021
-
 (2E)-oct-2-enoyl-CoA pH 8, 30°C, L65A mutant Aeromonas caviae
4.2.1.119 0.024
-
 crotonyl-CoA pH 8, 30°C, wild-type Aeromonas caviae
4.2.1.119 0.027
-
 dec-2-enoyl-CoA pH 8, 30°C, L65A mutant Aeromonas caviae
4.2.1.119 0.033
-
 crotonyl-CoA pH 8, 30°C, L65A mutant Aeromonas caviae
4.2.1.119 0.034
-
 dodec-2-enoyl-CoA pH 8, 30°C, L65A mutant Aeromonas caviae
4.2.1.119 0.04
-
 hexenoyl-CoA pH 8, 30°C, wild-type Aeromonas caviae
4.2.1.119 0.042
-
 dec-2-enoyl-CoA pH 8, 30°C, wild-type Aeromonas caviae
4.2.1.119 0.042
-
 (2E)-oct-2-enoyl-CoA pH 8, 30°C, wild-type Aeromonas caviae
4.2.1.119 0.043
-
 dodec-2-enoyl-CoA pH 8, 30°C, wild-type Aeromonas caviae
4.2.1.119 0.076
-
 (2E)-oct-2-enoyl-CoA pH 8, 30°C, V130G mutant Aeromonas caviae
4.2.1.119 0.102
-
 hexenoyl-CoA pH 8, 30°C, V130G mutant Aeromonas caviae
4.2.1.119 0.154
-
 crotonyl-CoA pH 8, 30°C, V130G mutant Aeromonas caviae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Aeromonas caviae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.119 0.05
-
 L654G/V130G mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 0.08
-
 L65A/V130G mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 0.55
-
 S62A mutant with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 0.86
-
 wild-type with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 1.98
-
 L65G mutant with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 2.25
-
 L65I mutant with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 6.59
-
 V130A mutant with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 7.92
-
 L65V mutant with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 15.8
-
 L65G mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 21.2
-
 V130G mutant with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 67
-
 S62A mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 68.5
-
 V130G mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 69.8
-
 L65A mutant with octenoyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 883
-
 V130G mutant with crotonyl-CoA as substrate, purified enzyme Aeromonas caviae
4.2.1.119 1256
-
 L65A mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 1288
-
 V130A mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 1538
-
 L65V mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 1594
-
 wild-type with crotonyl-CoA as substrate, cell extract Aeromonas caviae
4.2.1.119 1880
-
 L65I mutant with crotonyl-CoA as substrate, cell extract Aeromonas caviae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 crotonyl-CoA + H2O
-
 Aeromonas caviae 3-hydroxybutanoyl-CoA
-
 ?
4.2.1.119 dec-2-enoyl-CoA + H2O
-
 Aeromonas caviae 3-hydroxydecanoyl-CoA
-
 ?
4.2.1.119 dodec-2-enoyl-CoA + H2O
-
 Aeromonas caviae 3-hydroxydodecanoyl-CoA
-
 ?
4.2.1.119 hexenoyl-CoA + H2O
-
 Aeromonas caviae 3-hydroxyhexanoyl-CoA
-
 ?
4.2.1.119 oct-2-enoyl-CoA + H2O
-
 Aeromonas caviae 3-hydroxyoctanoyl-CoA
-
 ?
4.2.1.119 trans-2-octenoyl-CoA + H2O
-
 Aeromonas caviae 3-hydroxyoctanoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.119 PhaJAc
-
 Aeromonas caviae