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Literature summary extracted from

  • Konishi, J.; Maruhashi, K.
    2-(2'-Hydroxyphenyl)benzene sulfinate desulfinase from the thermophilic desulfurizing bacterium Paenibacillus sp. strain A11-2: purification and characterization (2003), Appl. Microbiol. Biotechnol., 62, 356-361.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.13.1.3 Mg2+ 1.23fold activation at 1 mM Paenibacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.3 gene tdsB, DNA sequence determination, overexpression in Escherichia coli BL21 (DE3) Paenibacillus sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.13.1.3 C17S site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant Paenibacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.1.3 2-Hydroxybiphenyl 30% inhibition at 5 mM, the OH-group is important for the inhibitory effect Paenibacillus sp.
3.13.1.3 Cu2+ 67% inhibition at 1 mM Paenibacillus sp.
3.13.1.3 DTNB 86% inhibition at 0.1 mM Paenibacillus sp.
3.13.1.3 Fe2+ 44% inhibition at 1 mM Paenibacillus sp.
3.13.1.3 iodoacetic acid 95% inhibition at 1 mM Paenibacillus sp.
3.13.1.3 Mn2+ 71% inhibition at 1 mM Paenibacillus sp.
3.13.1.3 additional information no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl Paenibacillus sp.
3.13.1.3 N-ethylmaleimide complete inhibition at 1 mM Paenibacillus sp.
3.13.1.3 Ni2+ 24% inhibition at 1 mM Paenibacillus sp.
3.13.1.3 o-hydroxystyrene product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect Paenibacillus sp.
3.13.1.3 p-chloromercuribenzoate 92% inhibition at 0.1 mM Paenibacillus sp.
3.13.1.3 Zn2+ 98% inhibition at 1 mM Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.3 0.33
-
2-(2-hydroxyphenyl)benzenesulfinate pH 7.0, 50°C Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.1.3 31000
-
gel filtration Paenibacillus sp.
3.13.1.3 39000
-
1 * 39000, SDS-PAGE Paenibacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.3 2-(2'-hydroxyphenyl)benzenesulfinate + H2O Paenibacillus sp. final step of the desulfurozation of dibenzothiophene 2-hydroxybiphenyl + sulfite
-
ir
3.13.1.3 2-(2'-hydroxyphenyl)benzenesulfinate + H2O Paenibacillus sp. A11-2 final step of the desulfurozation of dibenzothiophene 2-hydroxybiphenyl + sulfite
-
ir

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.3 Paenibacillus sp.
-
-
-
3.13.1.3 Paenibacillus sp. A11-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.3 native enzyme from strain A11-2 63fold Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.1.3 0.139
-
purified enzyme Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.3 2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
Paenibacillus sp. 2-hydroxybiphenyl + sulfite
-
ir
3.13.1.3 2-(2'-hydroxyphenyl)benzenesulfinate + H2O final step of the desulfurozation of dibenzothiophene Paenibacillus sp. 2-hydroxybiphenyl + sulfite
-
ir
3.13.1.3 2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
Paenibacillus sp. A11-2 2-hydroxybiphenyl + sulfite
-
ir
3.13.1.3 2-(2'-hydroxyphenyl)benzenesulfinate + H2O final step of the desulfurozation of dibenzothiophene Paenibacillus sp. A11-2 2-hydroxybiphenyl + sulfite
-
ir
3.13.1.3 2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
Paenibacillus sp. o-hydroxystyrene + sulfite
-
?
3.13.1.3 2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
Paenibacillus sp. A11-2 o-hydroxystyrene + sulfite
-
?

Subunits

EC Number Subunits Comment Organism
3.13.1.3 monomer 1 * 39000, SDS-PAGE Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.13.1.3 2-(2'-hydroxyphenyl)benzene sulfinate desulfinase
-
Paenibacillus sp.
3.13.1.3 TdsB
-
Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.1.3 55
-
-
Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.1.3 55
-
30 min, stable up to Paenibacillus sp.
3.13.1.3 60
-
rapid loss of activity Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.1.3 8
-
-
Paenibacillus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.1.3 additional information no cofactors required Paenibacillus sp.