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Literature summary extracted from

  • Shimojima, M.; Benning, C.
    Native uridine 5'-diphosphate-sulfoquinovose synthase, SQD1, from spinach purified as a 250-kDa complex (2003), Arch. Biochem. Biophys., 413, 123-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.1 cDNA isolation and functional expression in Escherichia coli Spinacia oleracea
3.13.1.1 expression in Escherichia coli Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.1 additional information
-
 additional information Km values for UDP-glucose are indistinguishable for the native and the recombinant enzyme, but the Km value for sulfite is over 4fold lower for the native enzyme Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.13.1.1 chloroplast
-
 Spinacia oleracea 9507
-
3.13.1.1 chloroplast native SoSQD1 is primarily present in the stroma Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.1.1 46700
-
 x * 46700, calculated from sequence Spinacia oleracea
3.13.1.1 250000
-
 gel filtration Spinacia oleracea
3.13.1.1 250000
-
 native enzyme in a complex with accessory proteins, gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.1 UDP-glucose + sulfite Spinacia oleracea key enzyme in formation of the sulfolipid head group precursor UDP-sulfoquinovose, sulfoquinovosyldiacylglycerol is a polar lipid present in photosynthetic membranes, enzyme is complexed in vivo with accessory proteins UDP-6-sulfoquinovose + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.1 Spinacia oleracea Q84KI6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.1
-
 Spinacia oleracea
3.13.1.1 native enzyme from chloroplasts, recombinant enzyme from Escherichia coli Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
3.13.1.1 UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite required NAD+ appears to oxidize the substrate to UDP-4-dehydroglucose, which dehydrates to UDP-4-dehydro-6-deoxygluc-5-enose, to which sulfite can add, the reaction is completed when the substrate is rehydrogenated at C-4 Spinacia oleracea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.13.1.1 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.1 UDP-glucose + sulfite
-
 Spinacia oleracea UDP-6-sulfoquinovose + H2O
-
 ?
3.13.1.1 UDP-glucose + sulfite key enzyme in formation of the sulfolipid head group precursor UDP-sulfoquinovose, sulfoquinovosyldiacylglycerol is a polar lipid present in photosynthetic membranes, enzyme is complexed in vivo with accessory proteins Spinacia oleracea UDP-6-sulfoquinovose + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
3.13.1.1 ? x * 46700, calculated from sequence Spinacia oleracea
3.13.1.1 dimer homodimer Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
3.13.1.1 SoSQD1
-
 Spinacia oleracea
3.13.1.1 SQD1
-
 Spinacia oleracea
3.13.1.1 sulfite:UDP-glucose sulfotransferase
-
 Spinacia oleracea
3.13.1.1 UDPsulfoquinovose synthase
-
 Spinacia oleracea
3.13.1.1 uridine 5'-diphosphate-sulfoquinovose synthase
-
 Spinacia oleracea