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Literature summary extracted from

  • Yang, K.W.; Sobieski, D.N.; Carenbauer, A.L.; Crawford, P.A.; Makaroff, C.A.; Crowder, M.W.
    Explaining the inhibition of glyoxalase II by 9-fluorenylmethoxycarbonyl-protected glutathione derivatives (2003), Arch. Biochem. Biophys., 414, 271-278.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.6 K65A site-directed mutagenesis, reduced sensitivity to 9-fluoromethoxycarbonyl- or phenylmethoxycarbonyl-protected glutathione derivative inhibitors Arabidopsis thaliana
3.1.2.6 L9A site-directed mutagenesis, reduced sensitivity to 9-fluoromethoxycarbonyl- or phenylmethoxycarbonyl-protected glutathione derivative inhibitors Arabidopsis thaliana
3.1.2.6 W57D site-directed mutagenesis, reduced sensitivity to 9-fluoromethoxycarbonyl- or phenylmethoxycarbonyl-protected glutathione derivative inhibitors Arabidopsis thaliana
3.1.2.6 W57N site-directed mutagenesis, reduced sensitivity to 9-fluoromethoxycarbonyl- or phenylmethoxycarbonyl-protected glutathione derivative inhibitors Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 N,S-bis-(9-fluorenylmethoxycarbonyl)glutathione
-
 Arabidopsis thaliana
3.1.2.6 N,S-bis-(phenylmethoxycarbonyl)glutathione
-
 Arabidopsis thaliana
3.1.2.6 N-(9-fluorenylmethoxycarbonyl)-S-phenylmethoxycarbonylglutathione
-
 Arabidopsis thaliana
3.1.2.6 N-(9-fluorenylmethoxycarbonyl)glutathione
-
 Arabidopsis thaliana
3.1.2.6 N-phenylmethoxycarbonyl-S-(9-fluorenylmethoxycarbonyl)glutathione
-
 Arabidopsis thaliana
3.1.2.6 N-phenylmethoxycarbonylglutathione
-
 Arabidopsis thaliana
3.1.2.6 S-(9-fluorenylmethoxycarbonyl)glutathione
-
 Arabidopsis thaliana
3.1.2.6 S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione
-
 Arabidopsis thaliana
3.1.2.6 S-phenylmethoxycarbonylglutathione
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.151
-
 S-D-lactoylglutathione recombinant mutant L9A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.256
-
 S-D-lactoylglutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.268
-
 S-D-lactoylglutathione recombinant mutant W57D, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.288
-
 S-D-lactoylglutathione recombinant mutant K65A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.318
-
 S-D-lactoylglutathione recombinant mutant W57N, pH 7.2, 25°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Arabidopsis thaliana O24496
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Arabidopsis thaliana glutathione + D-lactate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 acetoacetylglutathione hydrolase
-
 Arabidopsis thaliana
3.1.2.6 Germ cell specific protein
-
 Arabidopsis thaliana
3.1.2.6 GLO II
-
 Arabidopsis thaliana
3.1.2.6 GLX2-2
-
 Arabidopsis thaliana
3.1.2.6 glyoxalase II
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, acetoacetylglutathione
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, hydroxyacylglutathione
-
 Arabidopsis thaliana
3.1.2.6 Round spermatid protein RSP29
-
 Arabidopsis thaliana
3.1.2.6 S-2-hydroxylacylglutathione hydrolase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 25
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.6 322
-
 S-D-lactoylglutathione recombinant mutant L9A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 370
-
 S-D-lactoylglutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 386
-
 S-D-lactoylglutathione recombinant mutant K65A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 421
-
 S-D-lactoylglutathione recombinant mutant W57N, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 426
-
 S-D-lactoylglutathione recombinant mutant W57D, pH 7.2, 25°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7.2
-
 assay at Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.6 0.00089
-
 N,S-bis-(9-fluorenylmethoxycarbonyl)glutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.0017
-
 N-(9-fluorenylmethoxycarbonyl)-S-phenylmethoxycarbonylglutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.002
-
 N-phenylmethoxycarbonyl-S-(9-fluorenylmethoxycarbonyl)glutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.0021
-
 N,S-bis-(9-fluorenylmethoxycarbonyl)glutathione recombinant mutant W57D, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.0023
-
 N,S-bis-(phenylmethoxycarbonyl)glutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.0027
-
 N,S-bis-(9-fluorenylmethoxycarbonyl)glutathione recombinant mutants W57N and K65A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.0028
-
 N,S-bis-(9-fluorenylmethoxycarbonyl)glutathione recombinant mutant L9A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.0049
-
 S-phenylmethoxycarbonylglutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.0056
-
 N-phenylmethoxycarbonylglutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.014
-
 S-(9-fluorenylmethoxycarbonyl)glutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.015
-
 N-(9-fluorenylmethoxycarbonyl)glutathione recombinant wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana