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Literature summary extracted from
- Purification and characterization of homodimeric methylmalonyl-CoA mutase from Sinorhizobium meliloti (2003), Arch. Microbiol., 180, 151-154.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Sinorhizobium meliloti |  |
| 5.4.99.2 | Li+ | slight inhibition at 3 mM | Sinorhizobium meliloti | |
| 5.4.99.2 | Na+ | slight inhibition at 3 mM | Sinorhizobium meliloti | |
| 5.4.99.2 | p-chloromercuribenzoate | - |
Sinorhizobium meliloti | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.2 | 0.00064 | - |
adenosylcobalamin | 37°C, pH 7, in presence of 3 mM NH4+ | Sinorhizobium meliloti | |
| 5.4.99.2 | 0.0086 | - |
adenosylcobalamin | 37°C, pH 7, without NH4+ | Sinorhizobium meliloti | |
| 5.4.99.2 | 0.11 | - |
(R,S)-methylmalonyl-CoA | 37°C, pH 7, in presence of 3 mM NH4+ | Sinorhizobium meliloti | |
| 5.4.99.2 | 0.13 | - |
(R,S)-methylmalonyl-CoA | 37°C, pH 7, without NH4+ | Sinorhizobium meliloti | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | NH4+ | stimulates at 3 mM | Sinorhizobium meliloti | |
| 5.4.99.2 | Rb+ | stimulates at 3 mM | Sinorhizobium meliloti | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 80000 | - |
alpha2, 2 * 80000, SDS-PAGE | Sinorhizobium meliloti |
| 5.4.99.2 | 165000 | - |
gel filtration | Sinorhizobium meliloti |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | Sinorhizobium meliloti | - |
succinyl-CoA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.2 | Sinorhizobium meliloti | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.2 | to homogeneity | Sinorhizobium meliloti |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 10.85 | - |
after UNO Q-1 purification | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Sinorhizobium meliloti | succinyl-CoA | - |
? | |
| 5.4.99.2 | methylmalonyl-CoA | - |
Sinorhizobium meliloti | succinyl-CoA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.2 | homodimer | alpha2, 2 * 80000, SDS-PAGE | Sinorhizobium meliloti |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.2 | MCM | - |
Sinorhizobium meliloti |
| 5.4.99.2 | methylmalonyl-CoA mutase | - |
Sinorhizobium meliloti |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 37 | - |
- |
Sinorhizobium meliloti |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 7 | - |
- |
Sinorhizobium meliloti |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | adenosylcobalamin | - |
Sinorhizobium meliloti | |
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