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Literature summary extracted from

  • Beuerle, T.; Pichersky, E.
    Purification and characterization of benzoate:coenzyme A ligase from Clarkia breweri (2002), ARCH. BIOCHEM. BIOPHYS., 400, 258-264.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.25 EDTA
-
 Clarkia breweri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.045
-
 benzoate pH 7.5, 22°C Clarkia breweri
6.2.1.25 0.095
-
 ATP pH 7.5, 22°C Clarkia breweri
6.2.1.25 0.13
-
 CoA pH 7.5, 22°C Clarkia breweri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.25 Co2+ restores activity partially after treatment with EDTA Clarkia breweri
6.2.1.25 Mg2+ restores activity after treatment with EDTA Clarkia breweri
6.2.1.25 Mn2+ restores activity after treatment with EDTA Clarkia breweri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.25 59000
-
 gel filtration Clarkia breweri
6.2.1.25 64500
-
 Comassie blue-stained gel filtration Clarkia breweri

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Clarkia breweri
-
 annual California plant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25 DEAE, anion-exchange, HAP, affinity on reactive red resin and anion-exchange on a Mono-Q chromatography Clarkia breweri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.25 0.07
-
 pH 7.5, 22°C Clarkia breweri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-aminobenzoate + CoA 50% of the activity relative to benzoate Clarkia breweri AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-hydroxybenzoate + CoA 15% of the activity relative to benzoate Clarkia breweri AMP + diphosphate + 2-hydroxybenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-hydroxybenzoate + CoA 10% of the activity relative to benzoate Clarkia breweri AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-hydroxy-3-methoxybenzoate + CoA 15% of the activity relative to benzoate Clarkia breweri AMP + diphosphate + 4-hydroxy-3-methoxybenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-hydroxybenzoate + CoA 25% of the activity relative to benzoate Clarkia breweri AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Clarkia breweri AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + phenylacetate + CoA 10% of the activity relative to benzoate Clarkia breweri AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.25 ATP + phenylpropionate + CoA 10% of the activity relative to benzoate Clarkia breweri AMP + diphosphate + phenylpropionyl-CoA
-
 ?
6.2.1.25 additional information no reaction with cinnamic, coumaric, ferulic, caffeic, p-aminobenzoic, octanoic, or 2,5-dihydroxybenzoic acid Clarkia breweri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.25 monomer 1 * 64500, gel filtration Clarkia breweri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.25 7.2 8.4
-
 Clarkia breweri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.25 6.4 9.2
-
 Clarkia breweri