EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.7.3 | korAB genes, expression in Escherichia coli | Hydrogenobacter thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.3 | 32678 | - |
x * 67673 (subunit alpha) + x * 32678 (subunit beta), calculated from sequence | Hydrogenobacter thermophilus |
1.2.7.3 | 33000 | - |
x * 68000 (alpha-subunit) + x * 33000 (beta-subunit), SDS-PAGE | Hydrogenobacter thermophilus |
1.2.7.3 | 67673 | - |
x * 67673 (subunit alpha) + x * 32678 (subunit beta), calculated from sequence | Hydrogenobacter thermophilus |
1.2.7.3 | 68000 | - |
x * 68000 (alpha-subunit) + x * 33000 (beta-subunit), SDS-PAGE | Hydrogenobacter thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus | involved in carbon dioxide fixation via a reductive TCA cycle | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus TK-6 / IAM 12695 | involved in carbon dioxide fixation via a reductive TCA cycle | succinyl-CoA + CO2 + reduced ferredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.3 | Hydrogenobacter thermophilus | Q9AJM0 and Q9AJL9 | Q9AJM0: alpha-subunit, Q9AJL9: beta-subunit | - |
1.2.7.3 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | Q9AJM0 and Q9AJL9 | Q9AJM0: alpha-subunit, Q9AJL9: beta-subunit | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.3 | wild-type and recombinant enzyme | Hydrogenobacter thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.7.3 | 0.68 | - |
70°C, pH not specified in the publication | Hydrogenobacter thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | involved in carbon dioxide fixation via a reductive TCA cycle | Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | activity of the recombinant enzyme with 2-oxobutyrate, 2-oxoisocaproate, 2-oxoisovalerate, oxalacetate, oxomalonate, pyruvate or phosphoenolpyruvate is less than 5% of the activity with 2-oxoglutarate | Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | involved in carbon dioxide fixation via a reductive TCA cycle | Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | activity of the recombinant enzyme with 2-oxobutyrate, 2-oxoisocaproate, 2-oxoisovalerate, oxalacetate, oxomalonate, pyruvate or phosphoenolpyruvate is less than 5% of the activity with 2-oxoglutarate | Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.3 | ? | x * 67673 (subunit alpha) + x * 32678 (subunit beta), calculated from sequence | Hydrogenobacter thermophilus |
1.2.7.3 | ? | x * 68000 (alpha-subunit) + x * 33000 (beta-subunit), SDS-PAGE | Hydrogenobacter thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.3 | OGOR | - |
Hydrogenobacter thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.3 | 70 | - |
assay at | Hydrogenobacter thermophilus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.7.3 | physiological function | involved in carbon dioxide fixation via a reductive TCA cycle | Hydrogenobacter thermophilus |