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Literature summary extracted from

  • Yun, N.R.; Arai, H.; Ishii, M.; Igarashi, Y.
    The genes for anabolic 2-oxoglutarate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6 (2001), Biochem. Biophys. Res. Commun., 282, 589-594.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.3 korAB genes, expression in Escherichia coli Hydrogenobacter thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.3 32678
-
x * 67673 (subunit alpha) + x * 32678 (subunit beta), calculated from sequence Hydrogenobacter thermophilus
1.2.7.3 33000
-
x * 68000 (alpha-subunit) + x * 33000 (beta-subunit), SDS-PAGE Hydrogenobacter thermophilus
1.2.7.3 67673
-
x * 67673 (subunit alpha) + x * 32678 (subunit beta), calculated from sequence Hydrogenobacter thermophilus
1.2.7.3 68000
-
x * 68000 (alpha-subunit) + x * 33000 (beta-subunit), SDS-PAGE Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin Hydrogenobacter thermophilus involved in carbon dioxide fixation via a reductive TCA cycle succinyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin Hydrogenobacter thermophilus TK-6 / IAM 12695 involved in carbon dioxide fixation via a reductive TCA cycle succinyl-CoA + CO2 + reduced ferredoxin
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.3 Hydrogenobacter thermophilus Q9AJM0 and Q9AJL9 Q9AJM0: alpha-subunit, Q9AJL9: beta-subunit
-
1.2.7.3 Hydrogenobacter thermophilus TK-6 / IAM 12695 Q9AJM0 and Q9AJL9 Q9AJM0: alpha-subunit, Q9AJL9: beta-subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.3 wild-type and recombinant enzyme Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.3 0.68
-
70°C, pH not specified in the publication Hydrogenobacter thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin involved in carbon dioxide fixation via a reductive TCA cycle Hydrogenobacter thermophilus succinyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin activity of the recombinant enzyme with 2-oxobutyrate, 2-oxoisocaproate, 2-oxoisovalerate, oxalacetate, oxomalonate, pyruvate or phosphoenolpyruvate is less than 5% of the activity with 2-oxoglutarate Hydrogenobacter thermophilus succinyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin involved in carbon dioxide fixation via a reductive TCA cycle Hydrogenobacter thermophilus TK-6 / IAM 12695 succinyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin activity of the recombinant enzyme with 2-oxobutyrate, 2-oxoisocaproate, 2-oxoisovalerate, oxalacetate, oxomalonate, pyruvate or phosphoenolpyruvate is less than 5% of the activity with 2-oxoglutarate Hydrogenobacter thermophilus TK-6 / IAM 12695 succinyl-CoA + CO2 + reduced ferredoxin
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.3 ? x * 67673 (subunit alpha) + x * 32678 (subunit beta), calculated from sequence Hydrogenobacter thermophilus
1.2.7.3 ? x * 68000 (alpha-subunit) + x * 33000 (beta-subunit), SDS-PAGE Hydrogenobacter thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.3 OGOR
-
Hydrogenobacter thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.3 70
-
assay at Hydrogenobacter thermophilus

General Information

EC Number General Information Comment Organism
1.2.7.3 physiological function involved in carbon dioxide fixation via a reductive TCA cycle Hydrogenobacter thermophilus