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Literature summary extracted from

  • Jordan, P.A.; Tang, Y.; Bradbury, A.J.; Thomson, A.J.; Guest, J.R.
    Biochemical and spectroscopic characterization of Escherichia coli aconitases (AcnA and AcnB) (1999), Biochem. J., 344, 739-746.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.3 additional information
-
 additional information two values are obtained for KM-value with isocitrate as substrate, depending on substrate concentration Escherichia coli
4.2.1.3 0.016
-
 cis-aconitate 20°C, pH 7.4, enzyme form AcnB Escherichia coli
4.2.1.3 0.058
-
 cis-aconitate 20°C, pH 7.4, enzyme form AcnA Escherichia coli
4.2.1.3 1.16
-
 citrate 20°C, pH 7.4, enzyme form AcnA Escherichia coli
4.2.1.3 11
-
 citrate 20°C, pH 7.4, enzyme form AcnB Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.3 Iron a significant proportion of the enzyme is in the inactive [3Fe-4S]1+ or apoenzyme forms. AcnB contains a much higher proportion of inactive enzyme than AcnA Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.3 Escherichia coli
-
 and strain JRG3171, contains two major aconitases, AcnA and AcnB
-
4.2.1.3 Escherichia coli JRG2387
-
 and strain JRG3171, contains two major aconitases, AcnA and AcnB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.3 AcnA and AcnB Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.3 cis-aconitate + H2O
-
 Escherichia coli ?
-
 ?
4.2.1.3 cis-aconitate + H2O
-
 Escherichia coli JRG2387 ?
-
 ?
4.2.1.3 citrate
-
 Escherichia coli cis-aconitate + H2O
-
 ?
4.2.1.3 citrate
-
 Escherichia coli JRG2387 cis-aconitate + H2O
-
 ?
4.2.1.3 isocitrate
-
 Escherichia coli cis-aconitate + H2O
-
 ?
4.2.1.3 isocitrate
-
 Escherichia coli JRG2387 cis-aconitate + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.3 7.5
-
 enzyme form AcnA and AcnB, the maximal activity of AcnB is nearly 2fold the activity of AcnA Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.3 5 10 pH 5.0: about 75% of maximal activity, pH 10.0: about 60% of maximal activity, AcnA Escherichia coli
4.2.1.3 6 9 pH 6.0: about 55% of maximal activity, pH 9.0: about 20% of maximal activity, AcnB Escherichia coli