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Literature summary extracted from
- Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans (2000), Biochem. J., 347, 431-439.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.5 | dithiothreitol | 10 mM, 50% inhibition of phospholipase activity | Cryptococcus neoformans |  |
| 3.1.1.5 | Fe3+ | 1 mM, 95% inhibition of phospholipase activity, 80% inhibition of lysophospholipase activity | Cryptococcus neoformans | |
| 3.1.1.5 | palmitoylcarnitine | 10 mM, 70% inhibition of phospholipase activity | Cryptococcus neoformans | |
| 3.1.1.5 | SDS | 0.05%, complete inhibition of phospholipase activity | Cryptococcus neoformans | |
| 3.1.1.5 | Triton X-100 | 50% inhibition of lysophospholipase activity | Cryptococcus neoformans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.5 | 0.092 | - |
1-palmitoyl-lysophosphatidylcholine | 37°C, pH 4.0 | Cryptococcus neoformans | |
| 3.1.1.5 | 0.19 | - |
1,2-dipalmitoylphosphatidylcholine | 37°C, pH 4.0 | Cryptococcus neoformans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.5 | extracellular | - |
Cryptococcus neoformans | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.5 | 140000 | 232000 | native PAGE | Cryptococcus neoformans |
| 3.1.1.5 | 160000 | 180000 | gel filtration | Cryptococcus neoformans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.5 | 2-lysophosphatidylcholine + H2O | Cryptococcus neoformans | - |
glycerophosphocholine + a carboxylate | - |
? | |
| 3.1.1.5 | phosphatidylcholine + H2O | Cryptococcus neoformans | - |
lysophosphatidylcholine + a carboxylate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.5 | Cryptococcus neoformans | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.1.5 | glycoprotein | - |
Cryptococcus neoformans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.5 | ammonium sulfate, phenyl-Sepharose, Uno Q-1, Superose 12 HR, Superdex 200 | Cryptococcus neoformans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.5 | 10.5 | - |
hydrolysis of 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans |
| 3.1.1.5 | 559 | - |
hydrolysis of 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.5 | 1,2-dioleoylphosphatidylcholine + H2O | 129% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | monooleoyl-lysophosphatidylcholine + oleic acid | - |
? | |
| 3.1.1.5 | 1,2-dioleoylphosphatidylethanolamine + H2O | - |
Cryptococcus neoformans | monooleoyl-lysophosphatidylethanolamine + oleic acid | 33.5% of activity with 1,2-dipalmitoylphosphatidylcholine | ? | |
| 3.1.1.5 | 1,2-dioleoylphosphatidylserine + H2O | - |
Cryptococcus neoformans | monooleoyl-lysophosphatidylserine + oleic acid | 22.5% of activity with 1,2-dipalmitoylphosphatidylcholine | ? | |
| 3.1.1.5 | 1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Cryptococcus neoformans | 1-palmitoyl-sn-glycero-3-phosphocholine + palmitate | - |
? | |
| 3.1.1.5 | 1,2-dipalmitoylphosphatidylcholine + H2O | - |
Cryptococcus neoformans | ? | - |
? | |
| 3.1.1.5 | 1-oleoyl-lysophosphatidylcholine + H2O | 194% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphorylcholine + oleic acid | - |
? | |
| 3.1.1.5 | 1-palmitoyl-2-oleoylphosphatidylcholine + H2O | 36% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | lysophosphatidylcholine + ? | - |
? | |
| 3.1.1.5 | 1-palmitoyl-lysophosphatidylcholine + H2O | - |
Cryptococcus neoformans | glycerophosphocholine + palmitic acid | - |
? | |
| 3.1.1.5 | 1-palmitoyl-lysophosphatidylethanolamine + H2O | 29% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphatidylethanolamine + palmitic acid | - |
? | |
| 3.1.1.5 | 1-palmitoyl-sn-glycero-3-phosphoserine + H2O | 3.8% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphoserine + palmitic acid | - |
? | |
| 3.1.1.5 | 2-lysophosphatidylcholine + H2O | - |
Cryptococcus neoformans | glycerophosphocholine + a carboxylate | - |
? | |
| 3.1.1.5 | lysophosphatidylinositol + H2O | 78% of activity with 1-palmitoyl-lysophosphatidylcholine | Cryptococcus neoformans | glycerophosphatidylinositol + a carboxylate | - |
? | |
| 3.1.1.5 | phosphatidylcholine + H2O | - |
Cryptococcus neoformans | lysophosphatidylcholine + a carboxylate | - |
? | |
| 3.1.1.5 | phosphatidylglycerol + H2O | 94.2% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | lysophosphatidylglycerol + a carboxylate | - |
? | |
| 3.1.1.5 | phosphatidylinositol + H2O | 15.6% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | lysophophatidylinositol + a carboxylate | - |
? | |
| 3.1.1.5 | platelet activating factor + H2O | 8.9% of activity with 1,2-dipalmitoylphosphatidylcholine | Cryptococcus neoformans | platelet activating factor + a carboxylate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.5 | ? | x * 70000-90000, SDS-PAGE | Cryptococcus neoformans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.5 | phospholipase B | - |
Cryptococcus neoformans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.5 | 20 | - |
lysophospholipase activity | Cryptococcus neoformans |
| 3.1.1.5 | 37 | - |
phospholipase activity | Cryptococcus neoformans |
| 3.1.1.5 | 40 | - |
transacylase activity | Cryptococcus neoformans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.5 | 50 | - |
45% loss of lysophospholipase and transacylase activity, complete loss of phospholipase activity | Cryptococcus neoformans |
| 3.1.1.5 | 60 | - |
complete loss of lysophospholipase and transacylase activity | Cryptococcus neoformans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.5 | 4 | - |
hydrolysis of phosphatidylcholin and lysophosphatidylcholin | Cryptococcus neoformans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.5 | 2.5 | 6 | approx. 50% of maximal phospholipase activity at pH 3.0 and pH 5.0, respectively | Cryptococcus neoformans |
| 3.1.1.5 | 2.5 | 7 | 50-60% of maximal lysophospholipase activity at pH 3.0 and pH 5.5, respectively | Cryptococcus neoformans |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.5 | Cryptococcus neoformans | 2D-electrophoresis | - |
3.5 |
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