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Literature summary extracted from

  • Lee, Y.S.; Cho, Y.D.
    Identification of essential active-site residues in ornithine decarboxylase of Nicotiana glutinosa decarboxylating both L-ornithine and L-lysine (2001), Biochem. J., 360, 657-665.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.17 expression in Escherichia coli Nicotiana glutinosa

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.17 C338A 14% of wild-type L-ornithine decarboxylase activity Nicotiana glutinosa
4.1.1.17 C377A 4% of wild-type L-ornithine decarboxylase activity Nicotiana glutinosa
4.1.1.17 C96A 42% of wild-type L-ornithine decarboxylase activity Nicotiana glutinosa
4.1.1.17 K95A 1% of wild-type L-ornithine decarboxylase activity Nicotiana glutinosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.17 5,5'-dithiobis(2-nitrobenzoic acid) 0.001 mM, approx. 80% inhibition of ODC activity after 8 min Nicotiana glutinosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.17 0.56
-
 L-ornithine
-
 Nicotiana glutinosa
4.1.1.17 1.59
-
 L-lysine
-
 Nicotiana glutinosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.17 46500
-
 2 * 46500, SDS-PAGE Nicotiana glutinosa
4.1.1.17 92000
-
 native PAGE Nicotiana glutinosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.17 L-lysine Nicotiana glutinosa
-
 1,5-diaminopentane + CO2
-
 ?
4.1.1.17 L-ornithine Nicotiana glutinosa
-
 putrescine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.17 Nicotiana glutinosa Q9FPK5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.17 recombinant ODC Nicotiana glutinosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.17 L-lysine
-
 Nicotiana glutinosa 1,5-diaminopentane + CO2
-
 ?
4.1.1.17 L-ornithine
-
 Nicotiana glutinosa putrescine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.17 dimer 2 * 46500, SDS-PAGE Nicotiana glutinosa

Synonyms

EC Number Synonyms Comment Organism
4.1.1.17 ODC
-
 Nicotiana glutinosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.17 40
-
 half-life 70 min Nicotiana glutinosa
4.1.1.17 50
-
 half-life 20 min Nicotiana glutinosa
4.1.1.17 60
-
 half-life 5 min Nicotiana glutinosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.17 0.24
-
 L-lysine
-
 Nicotiana glutinosa
4.1.1.17 77.8
-
 L-ornithine
-
 Nicotiana glutinosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.17 6.8
-
 L-lysine decarboxylation Nicotiana glutinosa
4.1.1.17 8
-
 L-ornithine decarboxylation Nicotiana glutinosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.17 6.5 7.5 L-lysine decarboxylation, approx. 40% of maximal activity at pH 7.5 Nicotiana glutinosa
4.1.1.17 7.2 8.7 L-ornithine decarboxylation, approx. 35% of maximal activity at pH 7.2 Nicotiana glutinosa