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Literature summary extracted from

  • St Maurice, M.; Bearne, S.L.
    Kinetics and thermodynamics of mandelate racemase catalysis (2002), Biochemistry, 41, 4048-4058.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.2.2 sucrose slight activating effect of sucrose on mutant enzyme efficiency. In presence of polymeric viscosogens poly(ethylene glycol) and Ficoll, no effect on turnover number or the ratio of turnover number and Km-value for the wild-type enzyme is observed Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
5.1.2.2 N197A slight activating effect of sucrose on mutant enzyme efficiency. In presewnce of polymeric viscosogens poly(ethylene glycol) and Ficoll, no effect on turnover number or the ratio of turnover number and Km-value for the wild-type enzyme is observed Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.1.2.2 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.2.2 (R)-mandelate
-
 Pseudomonas putida (S)-mandelate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.2.2 additional information
-
 additional information the turnover number of the mutant enzyme is unaffected by varying solvent viscosity Pseudomonas putida