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Literature summary extracted from

  • Tang, K.H.; Harms, A.; Frey, P.A.
    Identification of a novel pyridoxal 5'-phosphate binding site in adenosylcobalamin-dependent lysine 5,6-aminomutase from Porphyromonas gingivalis (2002), Biochemistry, 41, 8767-8776.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.3.3 expression in Escherichia coli Porphyromonas gingivalis

Protein Variants

EC Number Protein Variants Comment Organism
5.4.3.3 C235S 9% of wild-type activity Porphyromonas gingivalis
5.4.3.3 K144Q no activity Porphyromonas gingivalis
5.4.3.3 K23Q 75% of wild-type activity Porphyromonas gingivalis
5.4.3.3 K377Q 0.6% of wild-type activity Porphyromonas gingivalis
5.4.3.3 K446Q 25% of wild-type activity Porphyromonas gingivalis
5.4.3.3 K58Q 33% of wild-type activity Porphyromonas gingivalis
5.4.3.3 K90Q 72% of wild-type activity Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.3.3 (3S)-3,6-diaminohexanoate Porphyromonas gingivalis
-
 (3S,5S)-3,5-diaminohexanoate
-
 ?
5.4.3.3 D-lysine Porphyromonas gingivalis equilibrium constant of 1.2 2,5-diaminohexanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.3 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.3 recombinant 5,6-LAM Porphyromonas gingivalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.3.3 8.31
-
-
 Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.3 (3S)-3,6-diaminohexanoate
-
 Porphyromonas gingivalis (3S,5S)-3,5-diaminohexanoate
-
 ?
5.4.3.3 (3S)-3,6-diaminohexanoate 5,6-LAM has both beta-lysine 5,6-aminomutase and D-lysine 5,6-aminomutase activity Porphyromonas gingivalis (3S,5S)-3,5-diaminohexanoate
-
 ?
5.4.3.3 D-lysine equilibrium constant of 1.2 Porphyromonas gingivalis 2,5-diaminohexanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.3.3 5,6-LAM
-
 Porphyromonas gingivalis

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.3.3 adenosylcobalamin
-
 Porphyromonas gingivalis
5.4.3.3 pyridoxal 5'-phosphate
-
 Porphyromonas gingivalis