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Literature summary extracted from
- Engineering of substrate mimetics as novel-type substrates for glutamic acid-specific endopeptidases: design, synthesis, and application (2000), Biochim. Biophys. Acta, 1479, 114-122.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.82 | synthesis | peptide coupling catalyzed by proteasesis an alternative to chemical solution and solid phase peptide synthesis | Bacillus licheniformis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.19 | additional information | - |
additional information | kinetics of the acyl transfer reactions with substrate mimetics | Staphylococcus aureus | |
| 3.4.21.19 | additional information | - |
additional information | kinetics of the acyl transfer reactions with substrate mimetics | Bacillus licheniformis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.19 | Bacillus licheniformis | - |
- |
- |
| 3.4.21.19 | Staphylococcus aureus | - |
- |
- |
| 3.4.21.82 | Bacillus licheniformis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.19 | further purification of commercial preparation | Bacillus licheniformis |
| 3.4.21.82 | - |
Bacillus licheniformis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.19 | commercial preparation | - |
Staphylococcus aureus | - |
| 3.4.21.19 | commercial preparation | - |
Bacillus licheniformis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | additional information | - |
hydrolysis activity of the enzyme with substrate mimetics | Staphylococcus aureus |
| 3.4.21.19 | additional information | - |
hydrolysis activity of the enzyme with substrate mimetics | Bacillus licheniformis |
| 3.4.21.19 | 360 | - |
- |
Bacillus licheniformis |
| 3.4.21.19 | 800 | - |
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.19 | acetyl-Glu-4-nitrophenyl + H2O | - |
Staphylococcus aureus | acetyl-Glu + 4-nitrophenol | - |
? |  |
| 3.4.21.19 | acetyl-Glu-4-nitrophenyl ester + H2O | - |
Bacillus licheniformis | acetyl-Glu + 4-nitrophenol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + 2-carboxyphenylthiol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Ala + 3-hydroxybenzoate | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + 3-carboxyphenol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Ala + 4-hydroxybenzoate | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + 4-carboxyphenol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala carboxyethylthioester + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala carboxyethylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + carboxyethylthiol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala carboxymethylthioester + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala carboxymethylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Ala + carboxymethylthiol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Asp methyl ester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Asp + methanol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Asp methyl ester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Asp + methanol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Glu methyl ester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-Glu + methanol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Glu methyl ester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Glu + methanol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Glu methylthioester + H2O | - |
Bacillus licheniformis | benzyloxycarbonyl-Glu + methylthiol | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Glu methylthioester + H2O | - |
Staphylococcus aureus | benzyloxycarbonyl-L-Glu + methylthiol | - |
? | |
| 3.4.21.19 | additional information | enzyme also performs acyl-transfer reactions with substrate mimetics carboxymethyl acylthioester, carboxyethyl acylthioester, 2-carboxyphenyl acylthioester, 3-carboxyphenyl acylester, 4-carboxyphenyl acylester | Staphylococcus aureus | ? | - |
? | |
| 3.4.21.19 | additional information | enzyme also performs acyl-transfer reactions with substrate mimetics carboxymethyl acylthioester, carboxyethyl acylthioester, 2-carboxyphenyl acylthioester, 3-carboxyphenyl acylester, 4-carboxyphenyl acylester | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.19 | N-tert-butyloxycarbonyl-L-Glu-alpha-phenyl ester + H2O | - |
Staphylococcus aureus | butyloxycarbonyl-L-Glu + phenol | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Gly-Leu-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Ile-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-Ala-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-GLy-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Met-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Nva-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Gly-Leu-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Ile-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Ala-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Gly-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Met-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Nva-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Gly-Leu-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Ile-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Ala-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Gly-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Met-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Nva-NH2 | - |
Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxyethyl thioester + Gly-Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxyethyl thioester + Ile-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Ala-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Gly-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxyethyl thioester + Met-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxyethyl thioester + Nva-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxymethyl thioester + Gly-Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxymethyl thioester + Ile-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Ala-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Gly-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxymethyl thioester + Met-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Ala-carboxymethyl thioester + Nva-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Asp-methyl ester + Gly-Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Asp-methyl ester + Ile-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Asp-methyl ester + Leu-Ala-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Asp-methyl ester + Leu-Gly-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Asp-methyl ester + Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Asp-methyl ester + Met-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Asp-methyl ester + Nva-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl ester + Gly-Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl ester + Ile-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl ester + Leu-Ala-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl ester + Leu-Gly-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl ester + Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl ester + Met-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl ester + Nva-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl thioester + Gly-Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl thioester + Ile-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl thioester + Leu-Ala-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl thioester + Leu-Gly-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl thioester + Leu-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl thioester + Met-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.82 | benzyloxycarbonyl-Glu-methyl thioester + Nva-NH2 | protease catalyzed acyltransfer from substrates to amino acid amides | Bacillus licheniformis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.19 | BL-GSE | - |
Bacillus licheniformis |
| 3.4.21.19 | Glu-specific endopeptidase | - |
Bacillus licheniformis |
| 3.4.21.19 | V8 protease | - |
Staphylococcus aureus |
| 3.4.21.82 | BL-GSE | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 37 | - |
assay at | Staphylococcus aureus |
| 3.4.21.19 | 37 | - |
assay at | Bacillus licheniformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 7.5 | - |
assay at | Bacillus licheniformis |
| 3.4.21.19 | 7.8 | - |
assay at | Staphylococcus aureus |
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