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Literature summary extracted from

  • Lombardo, B.; Raimo, G.; Bocchini, V.
    Molecular and functional properties of an archaeal phenylalanyl-tRNA synthetase from the hyperthermophile Sulfolobus solfataricus (2002), Biochim. Biophys. Acta, 1596, 246-252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.20 elongation factor 1alpha interaction enhances the activity Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 0.00048
-
 L-phenylalanine pH 7.8, 60°C Saccharolobus solfataricus
6.1.1.20 0.0015
-
 L-phenylalanine pH 7.8, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.20 56000
-
 2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE Saccharolobus solfataricus
6.1.1.20 64000
-
 2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE Saccharolobus solfataricus
6.1.1.20 240000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Saccharolobus solfataricus enzyme is essemtial for poly(Phe) synthesis AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.20 60fold Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.20 ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe tRNA channeling mechanism Saccharolobus solfataricus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.20 additional information
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe activity is restricted to endogenous tRNA, highly specific for L-phenylalanine Saccharolobus solfataricus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe enzyme is essemtial for poly(Phe) synthesis Saccharolobus solfataricus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 GTP + L-phenylalanine + tRNAPhe 40fold lower activity compared to ATP Saccharolobus solfataricus GMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 tetramer 2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 CML33
-
 Saccharolobus solfataricus
6.1.1.20 FRS
-
 Saccharolobus solfataricus
6.1.1.20 HSPC173
-
 Saccharolobus solfataricus
6.1.1.20 L-Phenylalanyl-tRNA synthetase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanine translase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanine--tRNA ligase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanyl transfer ribonucleic acid synthetase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanyl-transfer ribonucleate synthetase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanyl-transfer RNA ligase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanyl-transfer RNA synthetase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanyl-tRNA ligase
-
 Saccharolobus solfataricus
6.1.1.20 Phenylalanyl-tRNA synthetase
-
 Saccharolobus solfataricus
6.1.1.20 PheRS
-
 Saccharolobus solfataricus
6.1.1.20 Synthetase, phenylalanyl-transfer ribonucleate
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.20 65
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.20 50 92
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.20 88
-
 10 min, 50% inactivation Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.20 0.055
-
 L-phenylalanine pH 7.8, 60°C Saccharolobus solfataricus
6.1.1.20 0.115
-
 L-phenylalanine pH 7.8, 70°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.20 8
-
 broad maximum Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP not dependent on Saccharolobus solfataricus
6.1.1.20 GTP 40fold lower activity than with ATP Saccharolobus solfataricus