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Literature summary extracted from

  • Jinwal, U.K.; Roy, U.; Chowdhury, A.R.; Bhaduri, A.P.; Roy, P.K.
    Purification and characterization of an alkaline lipase from a newly isolated Pseudomonas mendocina PK-12CS and chemoselective hydrolysis of fatty acid ester (2003), Bioorg. Med. Chem., 11, 1041-1046.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.3 synthesis the enzyme performs chemically selective hydrolysis, synthesis of optically active substances from achiral or racemic compounds Pseudomonas mendocina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ba2+ 28% inhibition at 1 mM Pseudomonas mendocina
3.1.1.3 Cu2+ 52% inhibition at 1 mM Pseudomonas mendocina
3.1.1.3 diethyldicarbonate 65% inhibition at 0.1 mM, complete inhibition at 1 mM Pseudomonas mendocina
3.1.1.3 EDTA 38% inhibition at 1 mM Pseudomonas mendocina
3.1.1.3 Fe3+ 97% inhibition at 1 mM Pseudomonas mendocina
3.1.1.3 Hg2+ complete inhibition at 1 mM Pseudomonas mendocina
3.1.1.3 Mn2+ 17% inhibition at 1 mM Pseudomonas mendocina
3.1.1.3 additional information no inhibition by PMSF, no inhibition by Tween 20, Tween 80, and Triton X-100, negligible inhibition by Zn2+ Pseudomonas mendocina
3.1.1.3 SDS 95% inhibition at 0.4% Pseudomonas mendocina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular secretion to the culture medium Pseudomonas mendocina
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Mg2+ 20% activation at 1 mM Pseudomonas mendocina
3.1.1.3 additional information negligible stimulation by Na+ and Ca2+ at 1 mM Pseudomonas mendocina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 80000
-
 x * 80000, SDS-PAGE Pseudomonas mendocina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 additional information Pseudomonas mendocina Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource ?
-
 ?
3.1.1.3 additional information Pseudomonas mendocina PK-12CS Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource ?
-
 ?
3.1.1.3 tributyrin + H2O Pseudomonas mendocina
-
 dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O Pseudomonas mendocina PK-12CS
-
 dibutyrin + butyrate
-
 ?
3.1.1.3 triolein + H2O Pseudomonas mendocina
-
 diolein + oleate
-
 ?
3.1.1.3 triolein + H2O Pseudomonas mendocina PK-12CS
-
 diolein + oleate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 additional information the enzyme shows good stability in water-miscible and immiscible solvents, overview Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas mendocina
-
 alkaline lipase
-
3.1.1.3 Pseudomonas mendocina PK-12CS
-
 alkaline lipase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 from culture supernatant to homogeneity, 241fold Pseudomonas mendocina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.28
-
 purified enzyme Pseudomonas mendocina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview Pseudomonas mendocina ?
-
 ?
3.1.1.3 additional information Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource Pseudomonas mendocina ?
-
 ?
3.1.1.3 additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview Pseudomonas mendocina PK-12CS ?
-
 ?
3.1.1.3 additional information Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource Pseudomonas mendocina PK-12CS ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Pseudomonas mendocina dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O
-
 Pseudomonas mendocina PK-12CS dibutyrin + butyrate
-
 ?
3.1.1.3 trilinolin + H2O
-
 Pseudomonas mendocina dilinolin + linolate
-
 ?
3.1.1.3 triolein + H2O
-
 Pseudomonas mendocina diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Pseudomonas mendocina PK-12CS diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Pseudomonas mendocina dipalmitin + palmitate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Pseudomonas mendocina PK-12CS dipalmitin + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 80000, SDS-PAGE Pseudomonas mendocina

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 alkaline lipase
-
 Pseudomonas mendocina
3.1.1.3 PK-12CS lipase
-
 Pseudomonas mendocina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
-
 Pseudomonas mendocina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 22
-
 room temperature, stable for more than one month Pseudomonas mendocina
3.1.1.3 45
-
 1 h, loss of 50% activity Pseudomonas mendocina
3.1.1.3 57
-
 1 h, loss of 75% activity Pseudomonas mendocina
3.1.1.3 60
-
 half-life is 10 min Pseudomonas mendocina
3.1.1.3 65
-
 half-life is 4 min Pseudomonas mendocina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Pseudomonas mendocina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 7.6 8.6 37°C, activity drops sharply below pH 7.6 and above pH 8.6 Pseudomonas mendocina