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Literature summary extracted from

  • Fong, S.; Machajewski, T.D.; Mak, C.C.; Wong, C.
    Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars (2000), Chem. Biol., 7, 873-883.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.14
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.14 E9K/V118A KA1-4 variant, improved cleavage of D-2-dehydro-deoxygluconate Escherichia coli
4.1.2.14 G40A/V188A KA1-1 variant, improved cleavage of D-2-dehydro-deoxygluconate Escherichia coli
4.1.2.14 T84A/I92F KA1-2 variant, improved cleavage of D-2-dehydro-deoxygluconate Escherichia coli
4.1.2.14 T84A/I92F/V118A KA2 variant, improved kcat, Km and thermal stability Escherichia coli
4.1.2.14 T84A/I92F/V118A/E138V KA3 variant, small change of melting temperature Escherichia coli
4.1.2.14 T84A/I92F/V118A/G141S/T105I KA3-L2 variant, slightly less stable than wild-type Escherichia coli
4.1.2.14 T84A/I92F/V118A/T161A KA3-L1 variant, enhanced activity towards negatively charged glyoxylate and glyceraldehyde-3-phosphate Escherichia coli
4.1.2.14 V118A KA1-3 variant, improved cleavage of D-2-dehydro-deoxygluconate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.14 0.006
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA3-L1 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.03
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.03
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.15
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA1-2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.21
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA1-3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.35
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.46
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA3-L2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 2
-
 2-dehydro-3-deoxy-D-gluconate KA3-L1 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 12
-
 2-dehydro-3-deoxy-D-gluconate KA3-L2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 13
-
 2-dehydro-3-deoxy-D-gluconate KA3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 14
-
 2-dehydro-3-deoxy-D-gluconate KA2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 25
-
 2-dehydro-3-deoxy-D-gluconate KA1-2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 49
-
 2-dehydro-3-deoxy-D-gluconate KA1-3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 55
-
 2-dehydro-3-deoxy-D-gluconate KA1-4 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 65
-
 2-dehydro-3-deoxy-D-gluconate KA1-1 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 285
-
 2-dehydro-3-deoxy-D-gluconate wild-type enzyme, pH 7.5, 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-D-gluconate 6-phosphate Escherichia coli key enzyme in Entner-Doudoroff pathway pyruvate + D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.14
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-D-gluconate
-
 Escherichia coli pyruvate + D-glyceraldehyde
-
 ?
4.1.2.14 2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 Escherichia coli pyruvate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.14 2-dehydro-3-deoxy-D-gluconate 6-phosphate key enzyme in Entner-Doudoroff pathway Escherichia coli pyruvate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.14 D-2-dehydro-3-deoxygluconate poor substrate Escherichia coli pyruvate + D-glyceraldehyde
-
 ?
4.1.2.14 pyruvate + D-lactaldehyde
-
 Escherichia coli ?
-
 ?
4.1.2.14 pyruvate + DL-3-fluoro-2-hydroxypropanal
-
 Escherichia coli ?
-
 ?
4.1.2.14 pyruvate + DL-glyceraldehyde 3-phosphate
-
 Escherichia coli 2-dehydro-3-deoxy-D-gluconate
-
 ?
4.1.2.14 pyruvate + glycoaldehyde
-
 Escherichia coli ?
-
 ?
4.1.2.14 Pyruvate + glyoxylate
-
 Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.14 0.016
-
 2-dehydro-3-deoxy-D-gluconate wild-type enzyme, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.021
-
 2-dehydro-3-deoxy-D-gluconate KA3-L1 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.1
-
 2-dehydro-3-deoxy-D-gluconate KA1-1 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.11
-
 2-dehydro-3-deoxy-D-gluconate KA1-4 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.13
-
 2-dehydro-3-deoxy-D-gluconate KA1-3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.16
-
 2-dehydro-3-deoxy-D-gluconate KA1-2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.43
-
 2-dehydro-3-deoxy-D-gluconate KA3-L2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 0.58
-
 2-dehydro-3-deoxy-D-gluconate KA2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 1.09
-
 2-dehydro-3-deoxy-D-gluconate KA3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 2.5
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 6.08
-
 2-dehydro-3-deoxy-D-gluconate KA2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 6.08
-
 2-dehydro-3-deoxy-D-gluconate KA3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 7.8
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA3-L1 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 11.2
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA3-L2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 52
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 93
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA1-2 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 108
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate KA1-3 variant, pH 7.5, 25°C Escherichia coli
4.1.2.14 284
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli