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Literature summary extracted from

  • Walker, I.; Easton, C.J.; Ollis, D.L.
    Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase (2000), Chem. Commun. (Camb.), 2000, 671-672.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.45 C123S site-directed mutagenesis, mutation of the active site cysteine to a serine, giving a catalytic triad found in serine proteases, completely changes the catalytic activity to a dienelactone isomerase Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.45 additional information
-
 additional information
-
 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Pseudomonas sp.
-
 DLH type III
-
3.1.1.45 Pseudomonas sp. B13
-
 DLH type III
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate wild-type enzyme catalytic triad: Cys-His-Asn, mutant enzyme catalytic triad: Ser-His-Asn Pseudomonas sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 E/Z-dienelactone + H2O the reaction is reversible when performed by the mutant enzyme C123S Pseudomonas sp. maleylacetate
-
 ?
3.1.1.45 E/Z-dienelactone + H2O the reaction is reversible when performed by the mutant enzyme C123S Pseudomonas sp. B13 maleylacetate
-
 ?
3.1.1.45 additional information the mutant C123S catalyzes the isomerase reaction which changes the E-isomer to Z-isomer and vice versa Pseudomonas sp. ?
-
 ?
3.1.1.45 additional information the mutant C123S catalyzes the isomerase reaction which changes the E-isomer to Z-isomer and vice versa Pseudomonas sp. B13 ?
-
 ?
3.1.1.45 trans-dienelactone + H2O
-
 Pseudomonas sp. maleylacetate
-
 ?
3.1.1.45 trans-dienelactone + H2O
-
 Pseudomonas sp. B13 maleylacetate
-
 ?
3.1.1.45 Z-dienelactone + H2O
-
 Pseudomonas sp. maleyl acetate
-
 ?
3.1.1.45 Z-dienelactone + H2O
-
 Pseudomonas sp. B13 maleyl acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.45 dienelactone hydrolase
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.45 0.18
-
 Z-dienelactone mutant C123S, pH 7.0, 24°C Pseudomonas sp.
3.1.1.45 0.63
-
 E-dienelactone mutant C123S, pH 7.0, 24°C Pseudomonas sp.
3.1.1.45 6.08
-
 E-dienelactone mutant C123S, pH 7.0, 24°C Pseudomonas sp.
3.1.1.45 14
-
 E-dienelactone wild-type enzyme, pH 7.0, 24°C Pseudomonas sp.
3.1.1.45 19
-
 Z-dienelactone wild-type enzyme, pH 7.0, 24°C Pseudomonas sp.