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Literature summary extracted from

  • Colombo, S.; D'Auria, S.; Fusi, P.; Zecca, L.; Raia, C.A.; Tortora, P.
    Purification and characterization of a thermostable carboxypeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus (1992), Eur. J. Biochem., 206, 349-357.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.17.B1 10% glycerol stabilizes Saccharolobus solfataricus
3.4.17.B1 13% remaining activity in 0.1% SDS at 70°C after 180 min, stable at 40°C Saccharolobus solfataricus
3.4.17.B1 40% remaining activity in 8 M urea after 55 min at 40°C Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.B1 EDTA complete inhibition, can be reversed by Zn2+ or to a lesser extent by Co2+ Saccharolobus solfataricus
3.4.17.B1 o-phenanthroline
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.B1 0.061
-
 benzyloxycarbonyl-L-Arg pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 0.17
-
 benzoyl-glycyl-arginine pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 0.22
-
 Benzyloxycarbonyl-Asp pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 0.23
-
 benzoyl-glycyl-L-lysine pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 0.31
-
 benzyloxycarbonyl-Phe pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 0.69
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 6.5, 60°C Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.17.B1 intracellular
-
 Saccharolobus solfataricus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.B1 Co2+ can partially substitute for Zn2+, activates Saccharolobus solfataricus
3.4.17.B1 additional information Ni2+, Mn2+, Ca2+, and Mg2+ are ineffective Saccharolobus solfataricus
3.4.17.B1 Zn2+ metalloprotease, dependent on Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.B1 42000
-
 4 * 42000, SDS-PAGE Saccharolobus solfataricus
3.4.17.B1 170000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.B1 peptide + H2O Saccharolobus solfataricus
-
 ?
-
 ?
3.4.17.B1 peptide + H2O Saccharolobus solfataricus P2
-
 ?
-
 ?
3.4.17.B1 protein + H2O Saccharolobus solfataricus
-
 peptides
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.17.B1 acetonitril 93% remaining activity in 50% acetonitril at 40°C after 101 min, inactivation at 70°C Saccharolobus solfataricus
3.4.17.B1 Ethanol 90% remaining activity in pure ethanol at 40°C after 98 min, inactivation over this time period at 70°C, 2% remaining activity in 50% ethanol at 70°C Saccharolobus solfataricus
3.4.17.B1 Methanol 56% remaining activity in pure methanol at 40°C after 98 min, inactivation over this time period at 70°C, 11% remaining activity in 50% methanol at 70°C Saccharolobus solfataricus
3.4.17.B1 tetrahydrofuran 19% remaining activity in 50% tetrahydrofuran after 42 min at 40°C, nactivation at 70°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.B1 Saccharolobus solfataricus P80092 strain MT-4, ATCC 49155
-
3.4.17.B1 Saccharolobus solfataricus P2 P80092 strain MT-4, ATCC 49155
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.B1 to electrophoretic homogeneity, 76fold Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.B1 additional information
-
 substrate specificity, esterase activity Saccharolobus solfataricus
3.4.17.B1 4.1
-
 purified enzyme, substrate benzyloxycarbonyl-Phe Saccharolobus solfataricus
3.4.17.B1 8.6
-
 purified enzyme, substrate benzyloxycarbonyl-Asp Saccharolobus solfataricus
3.4.17.B1 16.3
-
 purified enzyme, substrate benzyloxycarbonyl-Arg Saccharolobus solfataricus
3.4.17.B1 21.9
-
 purified enzyme, substrate benzoyl-glycyl-arginine Saccharolobus solfataricus
3.4.17.B1 27.2
-
 purified enzyme, substrate benzoyl-glycyl-lysine Saccharolobus solfataricus
3.4.17.B1 38.7
-
 purified enzyme, substrate benzyloxycarbonyl-Gly-Gly-Phe Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.B1 benzoyl-glycyl-arginine + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzoyl-glycyl-L-lysine + H2O
-
 Saccharolobus solfataricus benzoyl-glycine + L-lysine
-
 ?
3.4.17.B1 benzoyl-glycyl-lysine + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Ala-Ser-methyl ester + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Ala-Val-methyl ester + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Arg + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Asp + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Glu-methyl ester + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Gly-Gly-Phe + H2O best substrate Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Gly-Gly-Phe + H2O best substrate Saccharolobus solfataricus P2 ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-L-Arg + H2O
-
 Saccharolobus solfataricus benzyloxycarbonate + L-Arg
-
 ?
3.4.17.B1 benzyloxycarbonyl-Leu-Ala-methyl ester + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Leu-Gly-methyl ester + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Phe + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Phe-Leu + H2O
-
 Saccharolobus solfataricus benzyloxycarbonyl-Phe + Leu
-
 ?
3.4.17.B1 benzyloxycarbonyl-Phe-Leu + H2O
-
 Saccharolobus solfataricus P2 benzyloxycarbonyl-Phe + Leu
-
 ?
3.4.17.B1 benzyloxycarbonyl-Tyr-ethyl ester + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 additional information enzyme also shows esterase activity, no activity with 4-nitroanilide substrates Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 additional information enzyme also shows esterase activity, no activity with 4-nitroanilide substrates Saccharolobus solfataricus P2 ?
-
 ?
3.4.17.B1 peptide + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 peptide + H2O broad substrate specificity, overview Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 peptide + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
3.4.17.B1 peptide + H2O broad substrate specificity, overview Saccharolobus solfataricus P2 ?
-
 ?
3.4.17.B1 protein + H2O
-
 Saccharolobus solfataricus peptides
-
 ?
3.4.17.B1 protein + H2O broad substrate specificity, overview Saccharolobus solfataricus peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.B1 tetramer 4 * 42000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.4.17.B1 carboxypeptidase S1
-
 Saccharolobus solfataricus
3.4.17.B1 M20.008 Merops-ID Saccharolobus solfataricus
3.4.17.B1 thermostable carboxypeptidase 1
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.B1 60
-
 assay at Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.17.B1 40 85 linear increase, still active at room temperature Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.B1
-
 25 instable at, glycerol stabilizes Saccharolobus solfataricus
3.4.17.B1 85
-
 stable for 15 min Saccharolobus solfataricus
3.4.17.B1 90
-
 85% remaining activity Saccharolobus solfataricus
3.4.17.B1 95
-
 17% remaining activity Saccharolobus solfataricus
3.4.17.B1 100
-
 inactivation Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.B1 0.97
-
 benzoyl-glycyl-arginine pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 3.4
-
 benzyloxycarbonyl-Phe pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 6
-
 Benzyloxycarbonyl-Asp pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 10.4
-
 Benzyloxycarbonyl-Arg pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 16.6
-
 benzoyl-glycyl-arginine pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 21.2
-
 benzoyl-glycyl-lysine pH 6.5, 60°C Saccharolobus solfataricus
3.4.17.B1 36.4
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 6.5, 60°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.B1 5.5 9 broad optimum, assay at pH 6.5 Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.17.B1 Saccharolobus solfataricus isoelectric focusing
-
 5.9