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Literature summary extracted from
- Purification and characterization of the first archaeal aconitase from the thermoacidophilic Sulfolobus acidocaldarius (2001), Eur. J. Biochem., 268, 1760-1771.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.3 | Fluorocitrate | - |
Sulfolobus acidocaldarius |  |
| 4.2.1.3 | trans-aconitate | - |
Sulfolobus acidocaldarius | |
| 4.2.1.3 | tricarballylate | - |
Sulfolobus acidocaldarius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.3 | 0.108 | - |
cis-aconitate | pH 8.4, 75°C | Sulfolobus acidocaldarius | |
| 4.2.1.3 | 0.37 | - |
isocitrate | pH 8.4, 75°C | Sulfolobus acidocaldarius | |
| 4.2.1.3 | 2.9 | - |
citrate | pH 8.4, 75°C | Sulfolobus acidocaldarius | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.3 | cytosol | - |
Sulfolobus acidocaldarius | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.3 | Iron | the enzyme hosts an interconvertible [3Fe-4S] cluster | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.3 | 96000 | - |
x * 96000, SDS-PAGE | Sulfolobus acidocaldarius |
| 4.2.1.3 | 145000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.3 | Sulfolobus acidocaldarius | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.2.1.3 | high oxygen sensitivity | Sulfolobus acidocaldarius |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.3 | - |
Sulfolobus acidocaldarius |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.3 | 55 | - |
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.3 | cis-aconitate + H2O | - |
Sulfolobus acidocaldarius | citrate | - |
? | |
| 4.2.1.3 | isocitrate | - |
Sulfolobus acidocaldarius | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.3 | ? | x * 96000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.3 | 75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.3 | 50 | 90 | 50°C: about 40% of maximal activity, 90°C: about 45% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.3 | 80 | - |
1 h, stable | Sulfolobus acidocaldarius |
| 4.2.1.3 | 95 | - |
half-life: 14 min | Sulfolobus acidocaldarius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.3 | 7.4 | - |
- |
Sulfolobus acidocaldarius |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.3 | 6.4 | 8.2 | pH 6.4: about 60% of maximal activity, pH 8.2: about 75% of maximal activity | Sulfolobus acidocaldarius |
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