EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.5 | additional information | activity is independent of the branched chain amino acids leucine, valine or isoleucine | Leuconostoc lactis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.5 | additional information | not inhibited by EDTA | Leuconostoc lactis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.5 | 1.3 | - |
(S)-2-Hydroxy-2-methyl-3-oxobutanoate | pH 6 | Leuconostoc lactis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.5 | additional information | activity is independent of metals | Leuconostoc lactis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 49000 | - |
2 * 49000, SDS-PAGE | Leuconostoc lactis |
4.1.1.5 | 100000 | - |
approximately, gel filtration | Leuconostoc lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.5 | additional information | Leuconostoc lactis | citrate metabolism | ? | - |
? | |
4.1.1.5 | additional information | Leuconostoc lactis NCW1 | citrate metabolism | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.5 | Leuconostoc lactis | - |
- |
- |
4.1.1.5 | Leuconostoc lactis NCW1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.5 | 5333fold | Leuconostoc lactis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | additional information | - |
- |
Leuconostoc lactis |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.1.5 | -70°C, purified, aliquoted ALD, 30% (v/v) glycerol, 3 weeks, stable | Leuconostoc lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.5 | (S)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Leuconostoc lactis | (R)-acetoin + CO2 | - |
? | |
4.1.1.5 | (S)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Leuconostoc lactis NCW1 | (R)-acetoin + CO2 | - |
? | |
4.1.1.5 | additional information | ALD does not catalyze the direct production of diacetyl from 2-acetolactate | Leuconostoc lactis | ? | - |
? | |
4.1.1.5 | additional information | citrate metabolism | Leuconostoc lactis | ? | - |
? | |
4.1.1.5 | additional information | ALD does not catalyze the direct production of diacetyl from 2-acetolactate | Leuconostoc lactis NCW1 | ? | - |
? | |
4.1.1.5 | additional information | citrate metabolism | Leuconostoc lactis NCW1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.5 | dimer | 2 * 49000, SDS-PAGE | Leuconostoc lactis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.5 | ALD | - |
Leuconostoc lactis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 60 | - |
stable up to | Leuconostoc lactis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.5 | 0.7 | - |
(S)-2-Hydroxy-2-methyl-3-oxobutanoate | pH 6 | Leuconostoc lactis | |
4.1.1.5 | 66.67 | - |
(S)-2-Hydroxy-2-methyl-3-oxobutanoate | pH 6 | Leuconostoc lactis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 6 | - |
- |
Leuconostoc lactis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.1.1.5 | Leuconostoc lactis | Mono P chromatofocusing | 4.4 | 4.3 |