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Literature summary extracted from

  • Uchida, H.; Kurakata, Y.; Sawamura, H.; Inamura, N.; Kotani, T.; Uwajima, T.
    Purification and properties of an esterase from Aspergillus nomius HS-1 degrading ethylene glycol dibenzoate (2003), FEMS Microbiol. Lett., 223, 123-127.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.1 biotechnology enzyme is an important biocatalyst for the preparation of a wide range of selectively protected and enantiomerically pure compounds Aspergillus nomiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 additional information not affected by EDTA, Ca2+, Mg2+, Co2+, Fe3+, Zn2+ Aspergillus nomiae
3.1.1.1 PMSF complete inhibition Aspergillus nomiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 additional information
-
 additional information kinetics Aspergillus nomiae
3.1.1.1 0.034
-
 4-nitrophenyl valerate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 0.06
-
 4-nitrophenyl butyrate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 0.075
-
 n-butyl benzoate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 0.11
-
 ethyl benzoate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 0.33
-
 4-nitrophenyl caproate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 0.35
-
 4-nitrophenyl propionate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 0.39
-
 ethyl 4-hydroxybenzoate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 0.66
-
 ethylene glycol dibenzoate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 1.2
-
 ethyl 3-hydroxybenzoate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 1.3
-
 4-nitrophenyl acetate pH 7.0, 37°C Aspergillus nomiae
3.1.1.1 2.3
-
 ethyl 3-methoxybenzoate pH 7.0, 37°C Aspergillus nomiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 extracellular secretion to the medium Aspergillus nomiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 additional information not affected by EDTA, Ca2+, Mg2+, Co2+, Fe3+, Zn2+ Aspergillus nomiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 60000
-
 gel filtration Aspergillus nomiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Aspergillus nomiae
-
-
-
3.1.1.1 Aspergillus nomiae HS-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 from culture medium to homogeneity, 28fold Aspergillus nomiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 135
-
 purified enzyme, substrate ethyl benzoate Aspergillus nomiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Aspergillus nomiae 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Aspergillus nomiae HS-1 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Aspergillus nomiae 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Aspergillus nomiae HS-1 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O
-
 Aspergillus nomiae 4-nitrophenol + caproate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O
-
 Aspergillus nomiae 4-nitrophenol + propionate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O
-
 Aspergillus nomiae HS-1 4-nitrophenol + propionate
-
 ?
3.1.1.1 4-nitrophenyl valerate + H2O
-
 Aspergillus nomiae 4-nitrophenol + valerate
-
 ?
3.1.1.1 ethyl 3-hydroxybenzoate + H2O
-
 Aspergillus nomiae ethanol + 3-hydroxybenzoate
-
 ?
3.1.1.1 ethyl 3-methoxybenzoate + H2O
-
 Aspergillus nomiae ethanol + 3-methoxybenzoate
-
 ?
3.1.1.1 ethyl 4-hydroxybenzoate + H2O
-
 Aspergillus nomiae ethanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 ethyl 4-methoxybenzoate + H2O
-
 Aspergillus nomiae ethanol + 4-methoxybenzoate
-
 ?
3.1.1.1 ethyl benzoate + H2O
-
 Aspergillus nomiae ethanol + benzoate
-
 ?
3.1.1.1 ethylene glycol dibenzoate + H2O
-
 Aspergillus nomiae ?
-
 ?
3.1.1.1 ethylene glycol dibenzoate + H2O
-
 Aspergillus nomiae HS-1 ?
-
 ?
3.1.1.1 additional information dramatic decline in activity with substrates of chain length above C6, no activity with ethyl esters of 2-hydroxy- and 2-methoxybenzoates, phenyl acetate, ferulate, and diethyl esters of terephthalate and phthalate Aspergillus nomiae ?
-
 ?
3.1.1.1 additional information dramatic decline in activity with substrates of chain length above C6, no activity with ethyl esters of 2-hydroxy- and 2-methoxybenzoates, phenyl acetate, ferulate, and diethyl esters of terephthalate and phthalate Aspergillus nomiae HS-1 ?
-
 ?
3.1.1.1 n-butyl benzoate + H2O
-
 Aspergillus nomiae n-butanol + benzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 60000, SDS-PAGE Aspergillus nomiae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 carboxyl esterase
-
 Aspergillus nomiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 50
-
-
 Aspergillus nomiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 40
-
 15 min, pH 7.0, loss of 5% activity Aspergillus nomiae
3.1.1.1 50
-
 15 min, pH 7.0, loss of 55% activity Aspergillus nomiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 6.5 7
-
 Aspergillus nomiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 4 9 80% activity at pH 4.0 and pH 9.0 Aspergillus nomiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Aspergillus nomiae isoelectric focusing
-
 4.8