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Literature summary extracted from

  • Pierik, A.J.; Ciceri, D.; Broker, G.; Edwards, C.H.; McFarlane, W.; Winter, J.; Buckel, W.; Golding, B.T.
    Rotation of the exo-methylene group of (R)-3-methylitaconate catalyzed by coenzyme B(12)-dependent 2-methyleneglutarate mutase from Eubacterium barkeri (2002), J. Am. Chem. Soc., 124, 14039-14048.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.4 overexpression in Escherichia coli Eubacterium barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.4 0.38
-
 (R)-3-[methyl-H3]methylitaconate pH 7.4 Eubacterium barkeri
5.4.99.4 0.4
-
 (R)-3-methylitaconate pH 7.4 Eubacterium barkeri
5.4.99.4 2.3
-
 2-methylene[4-H2]glutarate pH 7.4 Eubacterium barkeri
5.4.99.4 3.1
-
 2-Methyleneglutarate pH 7.4 Eubacterium barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.4 67000
-
 alpha4, 4 * 67000 Eubacterium barkeri

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.4 Eubacterium barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.4
-
 Eubacterium barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.4 (R)-3-[methyl-H3]methylitaconate
-
 Eubacterium barkeri 2-methyleneglutarate
-
 r
5.4.99.4 (R,Z)-3-methyl[2'-H1]itaconate
-
 Eubacterium barkeri 2-methyleneglutarate
-
 r
5.4.99.4 (R,Z)-3-[methyl-H3]methyl[2'-H1]itaconate
-
 Eubacterium barkeri 2-methyleneglutarate
-
 r
5.4.99.4 2-methyleneglutarate
-
 Eubacterium barkeri (R)-3-methylitaconate
-
 r
5.4.99.4 2-methylene[4-H2]glutarate
-
 Eubacterium barkeri (R)-3-methylitaconate
-
 r

Subunits

EC Number Subunits Comment Organism
5.4.99.4 tetramer alpha4, 4 * 67000 Eubacterium barkeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.4 1.1
-
 (R)-3-[methyl-H3]methylitaconate pH 7.4 Eubacterium barkeri
5.4.99.4 1.4
-
 (R,Z)-3-[methyl-H3]methyl[2'-H1]itaconate pH 7.4 Eubacterium barkeri
5.4.99.4 2.3
-
 2-methylene[4-H2]glutarate pH 7.4 Eubacterium barkeri
5.4.99.4 17
-
 (R,Z)-3-methyl[2'-H1]itaconate pH 7.4 Eubacterium barkeri
5.4.99.4 18
-
 (R)-3-methylitaconate pH 7.4 Eubacterium barkeri
5.4.99.4 30
-
 2-Methyleneglutarate pH 7.4 Eubacterium barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.99.4 adenosylcobalamin
-
 Eubacterium barkeri
5.4.99.4 cob(II)alamin
-
 Eubacterium barkeri