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Literature summary extracted from

  • Honer Zu Bentrup, K.; Miczak, A.; Swenson, D.L.; Russell, D.G.
    Characterization of activity and expression of isocitrate lyase in Mycobacterium avium and Mycobacterium tuberculosis (1999), J. Bacteriol., 181, 7161-7167.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 Icl and AceA are different open reading frames, both with isocitrate lyase activity Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
4.1.3.1 unstable, loses its activity within a couple of hours on ice, AceA enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 3-nitropropionate
-
 Mycobacterium tuberculosis
4.1.3.1 Bromopyruvate
-
 Mycobacterium tuberculosis
4.1.3.1 Itaconate
-
 Mycobacterium tuberculosis
4.1.3.1 itaconic anhydride
-
 Mycobacterium tuberculosis
4.1.3.1 malate Icl enzyme, 50% inhibition at 5 mM Mycobacterium tuberculosis
4.1.3.1 oxalate Icl enzyme, 50% inhibition at 5 mM Mycobacterium tuberculosis
4.1.3.1 succinate AceA enzyme, 50% inhibition at 5 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.145
-
 threo D-(s)-isocitrate Icl enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 1.3
-
 threo D-(s)-isocitrate AceA enzyme, Tricine buffer, 37°C, pH 7.5 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ Icl enzyme: most effective cation, optimal concentration: 5 mM Mycobacterium tuberculosis
4.1.3.1 Mn2+ Icl enzyme: can replace Mg2+, yielding 39% of the activity obtained with Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 50000
-
 calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Mycobacterium tuberculosis
-
 succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 1.3
-
 Icl enzyme Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
4.1.3.1 -80°C, stable for several months when frozen in liquid nitrogen and stored at -80°C, Icl enzyme Mycobacterium tuberculosis
4.1.3.1 4°C, after thawing and storage at 4°C the loss of activity after 2 months is minimal in the presence of glycerol and a reducing agent, Icl enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 (2R,3S)-isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 AceA
-
 Mycobacterium tuberculosis
4.1.3.1 ICL
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 6.8
-
 Icl enzyme Mycobacterium tuberculosis
4.1.3.1 7.5
-
 AceA enzyme Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.1 0.003
-
 3-nitropropionate Icl enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 0.11
-
 3-nitropropionate AceA enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 0.12
-
 Bromopyruvate Icl enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 0.12
-
 Itaconate Icl enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 0.19
-
 itaconic anhydride Icl enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 0.22
-
 Itaconate AceA enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 0.48
-
 itaconic anhydride AceA enzyme, 37°C Mycobacterium tuberculosis
4.1.3.1 0.71
-
 Bromopyruvate AceA enzyme, 37°C Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.3.1 Mycobacterium tuberculosis calculated from amino acid sequence
-
 5