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Literature summary extracted from

  • Nikodem, P.; Hecht, V.; Schlomann, M.; Pieper, D.H.
    New bacterial pathway for 4- and 5-chlorosalicylate degradation via 4-chlorocatechol and maleylacetate in Pseudomonas sp. strain MT1 (2003), J. Bacteriol., 185, 6790-6800.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.45 chlorocatechols growth on chlorocatechols induce the trans-dienelactone hydrolase activity by 6fold Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.45 Cu2+ 34% remaining activity after 5 min, 1 mM Pseudomonas sp.
3.1.1.45 EDTA 27% remaining activity after 5 min, 17% remaining activity after 60 min, 1 mM Pseudomonas sp.
3.1.1.45 Hg2+ 6% remaining activity after 5 min, 1 mM Pseudomonas sp.
3.1.1.45 additional information no inhibition by 4-chloromercuribenzoate Pseudomonas sp.
3.1.1.45 o-phenanthroline 55% remaining activity after 5 min, 18% remaining activity after 60 min, 1 mM Pseudomonas sp.
3.1.1.45 Zn2+ below 5% remaining activity after 5 min, 1 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.45 0.48
-
trans-dienelactone pH 7.5, 25°C Pseudomonas sp.
3.1.1.45 1.2
-
4-fluoromuconolactone pH 7.5, 25°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.45 39000
-
2 * 39000, SDS-PAGE Pseudomonas sp.
3.1.1.45 85000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 additional information Pseudomonas sp. antibiotic protoanemonin is no substrate ?
-
?
3.1.1.45 additional information Pseudomonas sp. MT1 antibiotic protoanemonin is no substrate ?
-
?
3.1.1.45 trans-dienelactone + H2O Pseudomonas sp. degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview maleylacetate
-
?
3.1.1.45 trans-dienelactone + H2O Pseudomonas sp. MT1 degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview maleylacetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas reinekei Q0VH44 gene salA
-
3.1.1.45 Pseudomonas sp.
-
possesses only the trans-substrate isomer-specific enzyme form
-
3.1.1.45 Pseudomonas sp. MT1
-
possesses only the trans-substrate isomer-specific enzyme form
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.1 culture condition:5-chlorosalicylate-grown cell
-
Pseudomonas reinekei
-
1.14.13.1 culture condition:salicylate-grown cell
-
Pseudomonas reinekei
-
1.14.13.1 additional information very low activity in succinate-grown cells Pseudomonas reinekei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.1 120
-
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 3-chlorosalicylate Pseudomonas reinekei
1.14.13.1 120
-
purified enzyme from salicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei
1.14.13.1 190
-
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 5-chlorosalicylate Pseudomonas reinekei
1.14.13.1 240
-
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 4-chlorosalicylate Pseudomonas reinekei
1.14.13.1 460
-
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei
3.1.1.45 190
-
purified enzyme, substrate 4-fluoromuconolactone Pseudomonas sp.
3.1.1.45 710
-
purified enzyme, substrate trans-dienelactone Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 3-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 3-chlorocatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 4-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 4-chlorocatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 5-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 5-chlorocatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 salicylate + NADH + H+ + O2
-
Pseudomonas reinekei catechol + NAD+ + H2O + CO2
-
?
3.1.1.45 4-fluoromuconolactone + H2O
-
Pseudomonas sp. maleylacetate + HF
-
?
3.1.1.45 4-fluoromuconolactone + H2O
-
Pseudomonas sp. MT1 maleylacetate + HF
-
?
3.1.1.45 additional information antibiotic protoanemonin is a poor substrate Pseudomonas sp. ?
-
?
3.1.1.45 additional information antibiotic protoanemonin is no substrate Pseudomonas sp. ?
-
?
3.1.1.45 additional information antibiotic protoanemonin is a poor substrate Pseudomonas sp. MT1 ?
-
?
3.1.1.45 additional information antibiotic protoanemonin is no substrate Pseudomonas sp. MT1 ?
-
?
3.1.1.45 trans-dienelactone + H2O very low activity with the cis-isomer Pseudomonas sp. maleylacetate
-
?
3.1.1.45 trans-dienelactone + H2O degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview Pseudomonas sp. maleylacetate
-
?
3.1.1.45 trans-dienelactone + H2O very low activity with the cis-isomer Pseudomonas sp. MT1 maleylacetate
-
?
3.1.1.45 trans-dienelactone + H2O degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview Pseudomonas sp. MT1 maleylacetate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.45 dimer 2 * 39000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 salicylate 1-hydroxylase
-
Pseudomonas reinekei
1.14.13.1 salicylate hydroxylase
-
Pseudomonas reinekei
1.14.13.1 SalOH
-
Pseudomonas reinekei
3.1.1.45 cis-dienelactone hydrolase
-
Pseudomonas sp.
3.1.1.45 cis-DLH
-
Pseudomonas sp.
3.1.1.45 dienelactone hydrolase
-
Pseudomonas sp.
3.1.1.45 DLH
-
Pseudomonas sp.
3.1.1.45 trans-dienelactone hydrolase
-
Pseudomonas sp.
3.1.1.45 trans-DLH
-
Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.1 25
-
assay at Pseudomonas reinekei
3.1.1.45 25
-
assay at Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.45 1630
-
4-fluoromuconolactone pH 7.5, 25°C Pseudomonas sp.
3.1.1.45 1633
-
4-fluoromuconolactone pH 7.5, 25°C Pseudomonas sp.
3.1.1.45 2650
-
trans-dienelactone pH 7.5, 25°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.45 7.5
-
assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 NADH
-
Pseudomonas reinekei

General Information

EC Number General Information Comment Organism
1.14.13.1 metabolism strain MT1 is capable of degrading 4- and 5-chlorosalicylates via 4-chlorocatechol, 3-chloromuconate, and maleylacetate by the chlorocatechol pathway, overview Pseudomonas reinekei