EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.45 | chlorocatechols | growth on chlorocatechols induce the trans-dienelactone hydrolase activity by 6fold | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.45 | Cu2+ | 34% remaining activity after 5 min, 1 mM | Pseudomonas sp. | |
3.1.1.45 | EDTA | 27% remaining activity after 5 min, 17% remaining activity after 60 min, 1 mM | Pseudomonas sp. | |
3.1.1.45 | Hg2+ | 6% remaining activity after 5 min, 1 mM | Pseudomonas sp. | |
3.1.1.45 | additional information | no inhibition by 4-chloromercuribenzoate | Pseudomonas sp. | |
3.1.1.45 | o-phenanthroline | 55% remaining activity after 5 min, 18% remaining activity after 60 min, 1 mM | Pseudomonas sp. | |
3.1.1.45 | Zn2+ | below 5% remaining activity after 5 min, 1 mM | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.45 | 0.48 | - |
trans-dienelactone | pH 7.5, 25°C | Pseudomonas sp. | |
3.1.1.45 | 1.2 | - |
4-fluoromuconolactone | pH 7.5, 25°C | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.45 | 39000 | - |
2 * 39000, SDS-PAGE | Pseudomonas sp. |
3.1.1.45 | 85000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.45 | additional information | Pseudomonas sp. | antibiotic protoanemonin is no substrate | ? | - |
? | |
3.1.1.45 | additional information | Pseudomonas sp. MT1 | antibiotic protoanemonin is no substrate | ? | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | Pseudomonas sp. | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | maleylacetate | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | Pseudomonas sp. MT1 | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | maleylacetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.1 | Pseudomonas reinekei | Q0VH44 | gene salA | - |
3.1.1.45 | Pseudomonas sp. | - |
possesses only the trans-substrate isomer-specific enzyme form | - |
3.1.1.45 | Pseudomonas sp. MT1 | - |
possesses only the trans-substrate isomer-specific enzyme form | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.1 | culture condition:5-chlorosalicylate-grown cell | - |
Pseudomonas reinekei | - |
1.14.13.1 | culture condition:salicylate-grown cell | - |
Pseudomonas reinekei | - |
1.14.13.1 | additional information | very low activity in succinate-grown cells | Pseudomonas reinekei | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.1 | 120 | - |
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 3-chlorosalicylate | Pseudomonas reinekei |
1.14.13.1 | 120 | - |
purified enzyme from salicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
1.14.13.1 | 190 | - |
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 5-chlorosalicylate | Pseudomonas reinekei |
1.14.13.1 | 240 | - |
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 4-chlorosalicylate | Pseudomonas reinekei |
1.14.13.1 | 460 | - |
purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
3.1.1.45 | 190 | - |
purified enzyme, substrate 4-fluoromuconolactone | Pseudomonas sp. |
3.1.1.45 | 710 | - |
purified enzyme, substrate trans-dienelactone | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.1 | 3-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 3-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 4-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 4-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 5-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 5-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | salicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | catechol + NAD+ + H2O + CO2 | - |
? | |
3.1.1.45 | 4-fluoromuconolactone + H2O | - |
Pseudomonas sp. | maleylacetate + HF | - |
? | |
3.1.1.45 | 4-fluoromuconolactone + H2O | - |
Pseudomonas sp. MT1 | maleylacetate + HF | - |
? | |
3.1.1.45 | additional information | antibiotic protoanemonin is a poor substrate | Pseudomonas sp. | ? | - |
? | |
3.1.1.45 | additional information | antibiotic protoanemonin is no substrate | Pseudomonas sp. | ? | - |
? | |
3.1.1.45 | additional information | antibiotic protoanemonin is a poor substrate | Pseudomonas sp. MT1 | ? | - |
? | |
3.1.1.45 | additional information | antibiotic protoanemonin is no substrate | Pseudomonas sp. MT1 | ? | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | very low activity with the cis-isomer | Pseudomonas sp. | maleylacetate | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | Pseudomonas sp. | maleylacetate | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | very low activity with the cis-isomer | Pseudomonas sp. MT1 | maleylacetate | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | Pseudomonas sp. MT1 | maleylacetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.45 | dimer | 2 * 39000, SDS-PAGE | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.1 | salicylate 1-hydroxylase | - |
Pseudomonas reinekei |
1.14.13.1 | salicylate hydroxylase | - |
Pseudomonas reinekei |
1.14.13.1 | SalOH | - |
Pseudomonas reinekei |
3.1.1.45 | cis-dienelactone hydrolase | - |
Pseudomonas sp. |
3.1.1.45 | cis-DLH | - |
Pseudomonas sp. |
3.1.1.45 | dienelactone hydrolase | - |
Pseudomonas sp. |
3.1.1.45 | DLH | - |
Pseudomonas sp. |
3.1.1.45 | trans-dienelactone hydrolase | - |
Pseudomonas sp. |
3.1.1.45 | trans-DLH | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.1 | 25 | - |
assay at | Pseudomonas reinekei |
3.1.1.45 | 25 | - |
assay at | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.45 | 1630 | - |
4-fluoromuconolactone | pH 7.5, 25°C | Pseudomonas sp. | |
3.1.1.45 | 1633 | - |
4-fluoromuconolactone | pH 7.5, 25°C | Pseudomonas sp. | |
3.1.1.45 | 2650 | - |
trans-dienelactone | pH 7.5, 25°C | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.45 | 7.5 | - |
assay at | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.1 | NADH | - |
Pseudomonas reinekei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.1 | metabolism | strain MT1 is capable of degrading 4- and 5-chlorosalicylates via 4-chlorocatechol, 3-chloromuconate, and maleylacetate by the chlorocatechol pathway, overview | Pseudomonas reinekei |