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Literature summary extracted from

  • Kulkarni, G.V.; Deobagkar, D.D.
    A cytosolic form of aminopeptidase P from Drosophila melanogaster: molecular cloning and characterization (2002), J. Biochem., 131, 445-452.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.9 DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.9 Ca2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
3.4.11.9 Co2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
3.4.11.9 Cu2+ 56% inhibition at 0.1 mM Drosophila melanogaster
3.4.11.9 EDTA 80% inhibition at 0.1 mM Drosophila melanogaster
3.4.11.9 Mg2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
3.4.11.9 Mn2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin Drosophila melanogaster
3.4.11.9 Ni2+ complete inhibition at 0.001 mM Drosophila melanogaster
3.4.11.9 Zn2+ complete inhibition at 0.001 mM Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.9 cytosol
-
 Drosophila melanogaster 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.9 Ca2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
3.4.11.9 Co2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
3.4.11.9 Mg2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
3.4.11.9 Mn2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin Drosophila melanogaster
3.4.11.9 additional information metalloprotease Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 71000
-
 x * 71000, recombinant His-tagged enzyme, SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Drosophila melanogaster O96794 cytosolic isoform
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.9 recombinant His-tagged enzyme from Escherichia coli Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.9 brain adult fly, high level Drosophila melanogaster
-
3.4.11.9 gut adult fly Drosophila melanogaster
-
3.4.11.9 additional information developmentally regulated Drosophila melanogaster
-
3.4.11.9 ovary adult fly Drosophila melanogaster
-
3.4.11.9 testis adult fly Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 bradykinin + H2O i.e. RPPGFSPFR, hydrolysis of the N-terminal Arg1-Pro2 bond Drosophila melanogaster Arg + des-Arg-bradykinin i.e. PPGFSPFR ?
3.4.11.9 substance P + H2O i.e. RPKPQQFFGLM, hydrolysis of the N-terminal Arg1-Pro2 bond Drosophila melanogaster Arg + des-Arg-substance P i.e. PKPQQFFGLM ?

Subunits

EC Number Subunits Comment Organism
3.4.11.9 ? x * 71000, recombinant His-tagged enzyme, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.4.11.9 aminopeptidase P
-
 Drosophila melanogaster
3.4.11.9 DAP-P
-
 Drosophila melanogaster
3.4.11.9 More the enzyme belongs to the peptidase family M24 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.9 37
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.9 7.6
-
 with substrate substance P Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.9 7.2 8
-
 Drosophila melanogaster