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Literature summary extracted from

  • Shariat-Madar, Z.; Mahdi, F.; Schmaier, A.H.
    Identification and characterization of prolylcarboxypeptidase as an endothelial cell prekallikrein activator (2002), J. Biol. Chem., 277, 17962-17969.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.2 angiotensin II IC50: 0.002 mM Homo sapiens
3.4.16.2 antipain IC50: 0.002 mM Homo sapiens
3.4.16.2 benzyloxycarbonyl-Pro-Pro-aldehyde dimethyl acetal IC50: 0.001 mM Homo sapiens
3.4.16.2 bradykinin IC50: 0.1 mM Homo sapiens
3.4.16.2 corn trypsin inhibitor IC50: 40 nM Homo sapiens
3.4.16.2 diisopropyl fluorophosphate 1 mM Homo sapiens
3.4.16.2 HgCl2 IC50: 0.5 mM Homo sapiens
3.4.16.2 leupeptin 0.1 mM Homo sapiens
3.4.16.2 additional information benzamidine has no effect at 10 mM Homo sapiens
3.4.16.2 N-(9-fluorenyl)methoxycarbonyl-Ala-Pyr-CN IC50: 50 nM Homo sapiens
3.4.16.2 NEM 3 mM, 30% inhibition Homo sapiens
3.4.16.2 phenylmethyl sulfonyl fluoride 3 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.16.2 0.0000067
-
 prekallikrein pH 7.1, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.16.2 CaCl2 optimal activity at 0.01 mM Homo sapiens
3.4.16.2 MgCl2 optimal activity at 1 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.16.2 umbilical vein endothelial cell line
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.16.2 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.2 prekallikrein + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.16.2 endothelial cell prekallikrein activator
-
 Homo sapiens
3.4.16.2 HUVEC PK activator
-
 Homo sapiens
3.4.16.2 PKA
-
 Homo sapiens
3.4.16.2 PRCP
-
 Homo sapiens
3.4.16.2 prolylcarboxypeptidase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.16.2 37
-
 half-life: 240 min Homo sapiens
3.4.16.2 95
-
 3 min, complete loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.16.2 7.1
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.16.2 6.8 7.4 more than 75% of maximal activity at pH 6.8 and pH 7.4 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.16.2 0.00004
-
 IC50: 40 nM Homo sapiens corn trypsin inhibitor
3.4.16.2 0.00005
-
 IC50: 50 nM Homo sapiens N-(9-fluorenyl)methoxycarbonyl-Ala-Pyr-CN
3.4.16.2 0.001
-
 IC50: 0.001 mM Homo sapiens benzyloxycarbonyl-Pro-Pro-aldehyde dimethyl acetal
3.4.16.2 0.002
-
 IC50: 0.002 mM Homo sapiens antipain
3.4.16.2 0.002
-
 IC50: 0.002 mM Homo sapiens angiotensin II
3.4.16.2 0.1
-
 IC50: 0.1 mM Homo sapiens bradykinin
3.4.16.2 0.5
-
 IC50: 0.5 mM Homo sapiens HgCl2