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Literature summary extracted from

  • Polderman-Tijmes, J.J.; Jekel, P.A.; Jeronimus-Stratingh, C.M.; Bruins, A.P.; Van Der Laan, J.M.; Sonke, T.; Janssen, D.B.
    Identification of the catalytic residues of alpha-amino acid ester hydrolase from Acetobacter turbidans by labeling and site-directed mutagenesis (2002), J. Biol. Chem., 277, 28474-28482.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.43 expression of His-tagged AEH in Escherichia coli Acetobacter pasteurianus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.43 D338A 0.07% of wild-type kcat Acetobacter pasteurianus
3.1.1.43 H370A 0.07% of wild-type kcat Acetobacter pasteurianus
3.1.1.43 H610A more than 69% of wild-type kcat, partially purified Acetobacter pasteurianus
3.1.1.43 R85A 67% of wild-type kcat Acetobacter pasteurianus
3.1.1.43 S156A 48% of wild-type kcat Acetobacter pasteurianus
3.1.1.43 S205A no activity Acetobacter pasteurianus
3.1.1.43 Y206A 44% of wild-type kcat Acetobacter pasteurianus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.43 p-nitrophenyl-p'-guanidino benzoate reversible inactivation Acetobacter pasteurianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.43 0.45
-
 cephalexin 30°C, pH 7.0 Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.43 an alpha-aminoacid ester + H2O Acetobacter pasteurianus
-
 an alpha-amino acid + an alcohol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.43 Acetobacter pasteurianus
-
 ATCC 9325
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.43 recombinant AEH, Ni2+-agarose, gel filtration Acetobacter pasteurianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.43 an alpha-aminoacid ester + H2O
-
 Acetobacter pasteurianus an alpha-amino acid + an alcohol
-
 ?
3.1.1.43 an alpha-aminoacid ester + H2O
-
 Acetobacter pasteurianus an alpha-amino acid + an alcohol
-
 r
3.1.1.43 cephalexin + H2O
-
 Acetobacter pasteurianus D-phenylglycine + 7-aminodesacetoxycephalosporanic acid
-
 r
3.1.1.43 p-nitrophenyl-p'-guanidino benzoate + H2O
-
 Acetobacter pasteurianus p-guanidino benzoate + p-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.43 AEH
-
 Acetobacter pasteurianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.43 274
-
 cephalexin 30°C, pH 7.0 Acetobacter pasteurianus