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Literature summary extracted from

  • Ito, M.; Tchoua, U.; Okamoto, M.; Tojo, H.
    Purification and properties of a phospholipase A2/lipase preferring phosphatidic acid, bis(monoacylglycerol) phosphate, and monoacylglycerol from rat testis (2002), J. Biol. Chem., 277, 43674-43681.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.23 (4-amidinophenyl) methanesulfonyl fluoride i.e. APMSF, inhibits at 0.5 mM Rattus norvegicus
3.1.1.23 methyl arachidonyl fluorophosphonate i.e. MAFP, inhibits at 0.05 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.23
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.23 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.23 8.21
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 bis(monoacylclycerol) phosphate + H2O at pH 5.5 Rattus norvegicus ?
-
 ?
3.1.1.23 lysobisphosphatidic acid + H2O at pH 5.5 Rattus norvegicus ?
-
 ?
3.1.1.23 monoarachidonoylglycerol + H2O
-
 Rattus norvegicus arachidonic acid + glycerol
-
 ?
3.1.1.23 monolinoleoylglycerol + H2O
-
 Rattus norvegicus linoic acid + glycerol
-
 ?
3.1.1.23 monooleoylglycerol + H2O
-
 Rattus norvegicus oleic acid + glycerol
-
 ?
3.1.1.23 monostearoylglycerol + H2O
-
 Rattus norvegicus stearic acid + glycerol
-
 ?
3.1.1.23 additional information enzyme exhibits both phosphatidic acid-preferring phospholipase A2 and monoacylglycerol lipase activities with a modest specificity towards unsaturated acyl chains, veryl low di- and triaclyglycerol lipase activity Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 5.5
-
 phospholipase A2 activity Rattus norvegicus
3.1.1.23 8
-
 lipase activity Rattus norvegicus