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Literature summary extracted from

  • Kojima, Y.; Shimizu, S.
    Purification and characterization of the lipase from Pseudomonas fluorescens HU380 (2003), J. Biosci. Bioeng., 96, 219-226.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.3 synthesis useful in application to the processing of industrial fats and oils containing eicosapentanoic acid and docosahexaenoic acid, such as fish oil splitting Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ 1.12fold activation at 1 mM, 1.36fold activation at 3 mM, 1.51fold activation at 10 mM Pseudomonas fluorescens
3.1.1.3 K+ slight activation Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 64000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas fluorescens
-
-
-
3.1.1.3 Pseudomonas fluorescens HU380
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 24.3fold Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 9854
-
 purified enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 methyl butyrate + H2O
-
 Pseudomonas fluorescens methanol + butyrate
-
 ?
3.1.1.3 methyl butyrate + H2O
-
 Pseudomonas fluorescens HU380 methanol + butyrate
-
 ?
3.1.1.3 methyl caprate + H2O best substrate Pseudomonas fluorescens methanol + caprate
-
 ?
3.1.1.3 methyl caproate + H2O
-
 Pseudomonas fluorescens methanol + caproate
-
 ?
3.1.1.3 methyl caprylate + H2O
-
 Pseudomonas fluorescens methanol + caprylate
-
 ?
3.1.1.3 methyl icosanoate + H2O
-
 Pseudomonas fluorescens methanol + icosanoate
-
 ?
3.1.1.3 methyl laurate + H2O
-
 Pseudomonas fluorescens methanol + laurate
-
 ?
3.1.1.3 methyl linoleate + H2O
-
 Pseudomonas fluorescens methanol + linoleate
-
 ?
3.1.1.3 methyl linolenate + H2O
-
 Pseudomonas fluorescens methanol + linolenate
-
 ?
3.1.1.3 methyl myristate + H2O
-
 Pseudomonas fluorescens methanol + myristate
-
 ?
3.1.1.3 methyl oleate + H2O
-
 Pseudomonas fluorescens methanol + oleate
-
 ?
3.1.1.3 methyl palmitate + H2O
-
 Pseudomonas fluorescens methanol + palmitate
-
 ?
3.1.1.3 methyl propionate + H2O
-
 Pseudomonas fluorescens methanol + propionate
-
 ?
3.1.1.3 methyl propionate + H2O
-
 Pseudomonas fluorescens HU380 methanol + propionate
-
 ?
3.1.1.3 methyl stearate + H2O
-
 Pseudomonas fluorescens methanol + stearate
-
 ?
3.1.1.3 additional information substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides Pseudomonas fluorescens ?
-
 ?
3.1.1.3 additional information substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides Pseudomonas fluorescens HU380 ?
-
 ?
3.1.1.3 olive oil + H2O emulsion Pseudomonas fluorescens ?
-
 ?
3.1.1.3 triacetin + H2O low activity Pseudomonas fluorescens diacetin + acetate
-
 ?
3.1.1.3 tributyrin + H2O best substrate Pseudomonas fluorescens dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O best substrate Pseudomonas fluorescens HU380 dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O best substrate Pseudomonas fluorescens dicaprin + caprate
-
 ?
3.1.1.3 tricaproin + H2O best substrate Pseudomonas fluorescens dicaproin + caproate
-
 ?
3.1.1.3 tricaprylin + H2O best substrate Pseudomonas fluorescens dicaprylin + caprylate
-
 ?
3.1.1.3 trilaurin + H2O
-
 Pseudomonas fluorescens dilaurin + laurate
-
 ?
3.1.1.3 trimyristin + H2O low activity Pseudomonas fluorescens dimyristin + myristate
-
 ?
3.1.1.3 triolein + H2O cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond Pseudomonas fluorescens diolein + oleate
-
 ?
3.1.1.3 triolein + H2O cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond Pseudomonas fluorescens HU380 diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O low activity Pseudomonas fluorescens dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O
-
 Pseudomonas fluorescens distearin + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 64000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 45
-
-
 Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 40
-
 70°C, stable for 60 min, in presence of 0.1% Triton X-100 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.5
-
-
 Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Pseudomonas fluorescens isoelectric focusing 4.8 4.7