EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | cloning of lipase gene lipA and its activator gene lipB, immediately downstream of lipA, DNA and amino acid sequences determination and analysis, overexpression of the insoluble protein in Escherichia coli BL21(DE3) with coexpression of lipB resulting in an active enzyme, enzyme expressed without lipB is not active | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Co2+ | 2 mM, pH 8.0 | Pseudomonas sp. | |
3.1.1.3 | Fe2+ | 2 mM, pH 8.0 | Pseudomonas sp. | |
3.1.1.3 | Hg2+ | 2 mM, pH 8.0 | Pseudomonas sp. | |
3.1.1.3 | Mg2+ | 2 mM, pH 8.0 | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ca2+ | - |
Pseudomonas sp. | |
3.1.1.3 | Na+ | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 31000 | - |
x * 31000, recombinant refolded enzyme, SDS-PAGE | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Pseudomonas sp. | Q9F0L9 | lipase LipA or PLS, gene lipA | - |
3.1.1.3 | Pseudomonas sp. SW-3 | Q9F0L9 | lipase LipA or PLS, gene lipA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | insoluble overexpressed recombinant enzyme from Escherichia coli, coeexpressed with activator LipB | Pseudomonas sp. |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.1.1.3 | insoluble recombinant enzyme is solubilized by 8 M urea, purified and refolded by removing urea in presence of Ca2+ | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 36.3 | - |
purified recombinant refolded enzyme, substrate 4-nitrophenyl palmitate | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl caprate + H2O | - |
Pseudomonas sp. | 4-nitrophenol + caprate | - |
? | |
3.1.1.3 | 4-nitrophenyl caprate + H2O | - |
Pseudomonas sp. SW-3 | 4-nitrophenol + caprate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | - |
Pseudomonas sp. | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | - |
Pseudomonas sp. SW-3 | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl myristate + H2O | - |
Pseudomonas sp. | 4-nitrophenol + myristate | - |
? | |
3.1.1.3 | 4-nitrophenyl myristate + H2O | - |
Pseudomonas sp. SW-3 | 4-nitrophenol + myristate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | best substrate | Pseudomonas sp. | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | best substrate | Pseudomonas sp. SW-3 | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl stearate + H2O | - |
Pseudomonas sp. | 4-nitrophenol + stearate | - |
? | |
3.1.1.3 | additional information | enzyme shows a preference for long-chain fatty acid triacylglyceride substrates | Pseudomonas sp. | ? | - |
? | |
3.1.1.3 | additional information | enzyme shows a preference for long-chain fatty acid triacylglyceride substrates | Pseudomonas sp. SW-3 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 31000, recombinant refolded enzyme, SDS-PAGE | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | lipase | - |
Pseudomonas sp. |
3.1.1.3 | More | the enzyme belongs to the lipase family I.1 | Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 50 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 50 | - |
stable, 90% remaining activity, 30 min | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 8 | - |
- |
Pseudomonas sp. |