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Literature summary extracted from

  • Simmons, A.J.; Rawls, J.M.; Piskur, J.; Davidson, J.N.
    A mutation that uncouples allosteric regulation of carbamyl phosphate synthetase in Drosophila (1999), J. Mol. Biol., 287, 277-285.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 additional information supressor of black mutation, Su(b), which causes a loss of UTP feedback inhibition, is a missense mutation resulting in a glutamate to lysine substitution within the second ATP binding site of enzyme Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UTP slight UTP inhibition of SU(b) mutant enzyme is probably due to the chelating of Mg2+ by UTP Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 0.04
-
 L-glutamine pH 7.4, 30°C, Su(b) mutant Drosophila melanogaster
6.3.5.5 0.057
-
 L-glutamine pH 7.4, 30°C, wild-type enzyme Drosophila melanogaster
6.3.5.5 2.4
-
 HCO3- pH 7.4, 30°C, Su(b) mutant Drosophila melanogaster
6.3.5.5 3
-
 HCO3- pH 7.4, 30°C, wild-type enzyme Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Drosophila melanogaster P05990 supressor of black mutation
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5 partial Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.5 additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Drosophila melanogaster 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
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