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Literature summary extracted from
- Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase (2000), Methods Enzymol., 324, 139-149.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.4 | dithiothreitol | activates 2fold at 5 mM | Pseudomonas mevalonii |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.3.4 | - |
Pseudomonas mevalonii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.4 | (R)-3-hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | |
| 4.1.3.4 | 1,10-phenanthroline | 43% activity after 4 h at 4 mM | Pseudomonas mevalonii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.4 | 0.02 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 8.2, 30°C, recombinant enzyme | Pseudomonas mevalonii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.4 | Cu2+ | tightly bound to the enzyme | Pseudomonas mevalonii | |
| 4.1.3.4 | Mg2+ | increases activity at millimolar concentrations | Pseudomonas mevalonii | |
| 4.1.3.4 | Mn2+ | increases activity at micromolar concentrations | Pseudomonas mevalonii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.4 | 64000 | - |
calculated from Stokes' radius | Pseudomonas mevalonii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.4 | additional information | Pseudomonas mevalonii | last step in ketogenesis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.4 | Pseudomonas mevalonii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.4 | - |
Pseudomonas mevalonii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.4 | 70 | - |
- |
Pseudomonas mevalonii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.3.4 | -80°C, retains full activity for several months in phosphate buffer containing 20% glycerol at pH 7.2 | Pseudomonas mevalonii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.4 | (S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | Acetyl-CoA + acetoacetate | - |
ir | |
| 4.1.3.4 | additional information | last step in ketogenesis | Pseudomonas mevalonii | ? | - |
? | |
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