EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.14.5 | 4-(2-aminoethyl)benzenesulfonyl fluoride | i.e. AEBSF | Hordeum vulgare | |
3.4.14.5 | Diprotin A | specific inhibition | Hordeum vulgare | |
3.4.14.5 | Diprotin B | specific inhibition | Hordeum vulgare | |
3.4.14.5 | additional information | no inhibition by EDTA, E-64, pepstatin A or leupeptin | Hordeum vulgare | |
3.4.14.5 | PMSF | - |
Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.14.5 | 0.000071 | - |
Lys-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00009 | - |
Arg-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00034 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00065 | - |
Phe-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.0007 | - |
Ile-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00073 | - |
Tyr-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00093 | - |
L-Thr-L-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00103 | - |
His-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00109 | - |
Leu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.0011 | - |
Pro-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00126 | - |
Ser-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00138 | - |
Gln-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00217 | - |
Met-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00298 | - |
Glu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00324 | - |
Asn-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00374 | - |
Lys-Ala-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00688 | - |
Gly-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 0.00888 | - |
Asp-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 85000 | - |
1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE | Hordeum vulgare |
3.4.14.5 | 105000 | - |
gel filtration | Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.14.5 | Hordeum vulgare | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.14.5 | glycoprotein | the deglycosylated enzyme is still active | Hordeum vulgare |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.14.5 | from green barley malt, more than 43000fold | Hordeum vulgare |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.14.5 | release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline | serine protease | Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.14.5 | seed | malt from green barley | Hordeum vulgare | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | additional information | - |
purified enzyme | Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.14.5 | Ala-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Ala-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Arg-Pro-7-amido-4-methylcoumarin + H2O | high activity | Hordeum vulgare | Arg-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Asn-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Asn-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Asp-Pro-7-amido-4-methylcoumarin + H2O | low activity | Hordeum vulgare | Asp-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | beta-casomorphin + H2O | cleavage sites: YP-/-FP-/-GP-/-IGP-/-RP | Hordeum vulgare | fragments | - |
? | |
3.4.14.5 | des-Pro2-bradykinin + H2O | cleavage sites: RP-/-GFSPFR | Hordeum vulgare | fragments | - |
? | |
3.4.14.5 | Gln-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Gln-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Glu-Pro-7-amido-4-methylcoumarin + H2O | low activity | Hordeum vulgare | Glu-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | His-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | His-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Ile-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Ile-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | L-Thr-L-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | L-Thr-L-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Leu-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Leu-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Lys-Ala-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Lys-Ala + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Lys-Pro-7-amido-4-methylcoumarin + H2O | best substrate | Hordeum vulgare | Lys-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Met-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Met-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | additional information | substrate specificity study, no activity with bradykinin, Pro-7-amido-4-methylcoumarin, benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin, and benzyloxycarbonyl-Phe-Ala-7-amido-4-methylcoumarin | Hordeum vulgare | ? | - |
? | |
3.4.14.5 | Phe-Pro-7-amido-4-methylcoumarin + H2O | low activity | Hordeum vulgare | Phe-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Pro-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Pro-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | Ser-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Ser-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.14.5 | substance P + H2O | cleavage sites: RP-/-KP-/-QQFFGLM | Hordeum vulgare | substance P peptide fragments | - |
? | |
3.4.14.5 | Tyr-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Tyr-Pro + 7-amino-4-methylcoumarin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.14.5 | monomer | 1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE | Hordeum vulgare |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.14.5 | dipeptidyl peptidase IV | - |
Hordeum vulgare |
3.4.14.5 | DPPIV | - |
Hordeum vulgare |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 25 | - |
assay at | Hordeum vulgare |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.14.5 | 1.8 | - |
Phe-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 4.6 | - |
Asp-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 10 | - |
Glu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 10 | - |
Gln-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 22 | - |
Leu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 23 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 35 | - |
Asn-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 41 | - |
His-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 41 | - |
Pro-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 58 | - |
Ser-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 60 | - |
Gly-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 67 | - |
Ile-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 71 | - |
Arg-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 90 | - |
Lys-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 92 | - |
Lys-Ala-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 99 | - |
Tyr-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 102 | - |
Met-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
3.4.14.5 | 117 | - |
L-Thr-L-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 7.2 | - |
- |
Hordeum vulgare |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.14.5 | 5.5 | 9 | - |
Hordeum vulgare |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.14.5 | Hordeum vulgare | isoelectric focusing | - |
3.6 |