Any feedback?
Literature summary extracted from
- Folate biosynthesis in higher plants. cDNA cloning, heterologous expression, and characterization of Dihydroneopterin aldolases (2004), Plant Physiol., 135, 103-111.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.25 | expressed in Escherichia coli | Arabidopsis thaliana |
| 4.1.2.25 | expressed in Escherichia coli | Solanum lycopersicum |
| 4.1.2.25 | expression in Escherichia coli | Solanum lycopersicum |
| 4.1.2.25 | expression in Escherichia coli | Arabidopsis thaliana |
| 5.1.99.8 | expression in Escherichia coli | Solanum lycopersicum |
| 5.1.99.8 | expression in Escherichia coli | Arabidopsis thaliana |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.25 | 13600 | - |
x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 4.1.2.25 | 14000 | - |
8 * 14000, gel filtration | Arabidopsis thaliana |
| 4.1.2.25 | 14100 | - |
x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
| 4.1.2.25 | 14600 | - |
8 * 14600, gel filtration | Solanum lycopersicum |
| 4.1.2.25 | 14600 | - |
x * 14600, SDS-PAGE | Solanum lycopersicum |
| 4.1.2.25 | 14685 | - |
x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 4.1.2.25 | 16300 | - |
x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
| 5.1.99.8 | 13600 | - |
x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 5.1.99.8 | 14100 | - |
x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
| 5.1.99.8 | 14600 | - |
x * 14600, SDS-PAGE | Solanum lycopersicum |
| 5.1.99.8 | 14685 | - |
x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 5.1.99.8 | 16300 | - |
x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.25 | Arabidopsis thaliana | - |
- |
- |
| 4.1.2.25 | Arabidopsis thaliana | Q9FM54 | bifunctional epimerase, EC 5.1.99.8, and aldolase | - |
| 4.1.2.25 | Arabidopsis thaliana | Q9SF23 | bifunctional epimerase, EC 5.1.99.8, and aldolase | - |
| 4.1.2.25 | Solanum lycopersicum | - |
- |
- |
| 5.1.99.8 | Arabidopsis thaliana | Q9FM54 | bifunctional epimerase and aldolase, EC 4.1.2.25 | - |
| 5.1.99.8 | Arabidopsis thaliana | Q9SF23 | bifunctional epimerase and aldolase, EC 4.1.2.25 | - |
| 5.1.99.8 | Solanum lycopersicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.25 | homogeneity | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.2.25 | leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.25 | 7,8-dihydromonapterin | - |
Solanum lycopersicum | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? |  |
| 4.1.2.25 | 7,8-dihydromonapterin | - |
Arabidopsis thaliana | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? | |
| 4.1.2.25 | 7,8-dihydroneopterin | - |
Arabidopsis thaliana | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? | |
| 4.1.2.25 | 7,8-dihydroneopterin | - |
Solanum lycopersicum | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? | |
| 4.1.2.25 | additional information | enzyme also mediates the epimerisation of 7,8-dihydroneopterin to 7,8-dihydromonapterin | Arabidopsis thaliana | ? | - |
? | |
| 4.1.2.25 | additional information | enzyme also mediates the epimerisation of 7,8-dihydroneopterin to 7,8-dihydromonapterin | Solanum lycopersicum | ? | - |
? | |
| 4.1.2.25 | additional information | enzyme catalyzes both the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin, reaction of EC 4.1.2.25, and the epimerization of 7,8-dihydroneopterin to 7,8-dihydromonapterin, it carries out the aldol cleavage reaction on the epimerization product, 7,8-dihydromonapterin, as well as on 7,8-dihydroneopterin | Solanum lycopersicum | ? | - |
? | |
| 4.1.2.25 | additional information | enzyme catalyzes both the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin, reaction of EC 4.1.2.25, and the epimerization of 7,8-dihydroneopterin to 7,8-dihydromonapterin, it carries out the aldol cleavage reaction on the epimerization product, 7,8-dihydromonapterin, as well as on 7,8-dihydroneopterin | Arabidopsis thaliana | ? | - |
? | |
| 5.1.99.8 | 7,8-dihydroneopterin | - |
Solanum lycopersicum | 7,8-dihydromonapterin | - |
? | |
| 5.1.99.8 | 7,8-dihydroneopterin | - |
Arabidopsis thaliana | 7,8-dihydromonapterin | - |
? | |
| 5.1.99.8 | additional information | enzyme catalyzes both the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin, reaction of EC 4.1.2.25, and the epimerization of 7,8-dihydroneopterin to 7,8-dihydromonapterin, it carries out the aldol cleavage reaction on the epimerization product, 7,8-dihydromonapterin, as well as on 7,8-dihydroneopterin | Solanum lycopersicum | ? | - |
? | |
| 5.1.99.8 | additional information | enzyme catalyzes both the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin, reaction of EC 4.1.2.25, and the epimerization of 7,8-dihydroneopterin to 7,8-dihydromonapterin, it carries out the aldol cleavage reaction on the epimerization product, 7,8-dihydromonapterin, as well as on 7,8-dihydroneopterin | Arabidopsis thaliana | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.25 | ? | x * 14600, SDS-PAGE | Solanum lycopersicum |
| 4.1.2.25 | ? | x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 4.1.2.25 | ? | x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
| 4.1.2.25 | octamer | 8 * 14000, gel filtration | Arabidopsis thaliana |
| 4.1.2.25 | octamer | 8 * 14600, gel filtration | Solanum lycopersicum |
| 5.1.99.8 | ? | x * 14600, SDS-PAGE | Solanum lycopersicum |
| 5.1.99.8 | ? | x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 5.1.99.8 | ? | x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.25 | At3g11750 | - |
Arabidopsis thaliana |
| 4.1.2.25 | At5g62980 | - |
Arabidopsis thaliana |
| 4.1.2.25 | FolB1 | - |
Solanum lycopersicum |
| 4.1.2.25 | FolB1 | - |
Arabidopsis thaliana |
| 4.1.2.25 | FolB2 | - |
Arabidopsis thaliana |
| 5.1.99.8 | At3g11750 | - |
Arabidopsis thaliana |
| 5.1.99.8 | At5g62980 | - |
Arabidopsis thaliana |
| 5.1.99.8 | FolB1 | - |
Solanum lycopersicum |
| 5.1.99.8 | FolB1 | - |
Arabidopsis thaliana |
| 5.1.99.8 | FolB2 | - |
Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
