Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kar, B.; Banerjee, R.; Bhattacharyya, B.C.
    Effect of additives on the behavioural properties of tannin acyl hydrolase (2003), Proc. Biochem., 38, 1285-1293.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 ammonium chloride 0.1% w/v, 4fold increased activity when used as nitrogen source Rhizopus arrhizus
3.1.1.20 ammonium nitrate 0.1% w/v, 1.2fold increased activity when used as nitrogen source Rhizopus arrhizus
3.1.1.20 ammonium sulfate with FeSO4, when used as nitrogen source, 1.7fold increase in activity Rhizopus arrhizus
3.1.1.20 Br- 1.6fold induction Rhizopus arrhizus
3.1.1.20 Hg+ slight activation at 1 mM, 105% activity Rhizopus arrhizus
3.1.1.20 Mg2+ activation at 1 mM, 137.9% activity Rhizopus arrhizus
3.1.1.20 S2O32- 2.2fold induction Rhizopus arrhizus
3.1.1.20 Tween 40 best at 0.4% v/v, above that concentration it inhibits the enzyme Rhizopus arrhizus
3.1.1.20 Tween 80 best at 0.05% v/v, above that concentration it inhibits the enzyme Rhizopus arrhizus
3.1.1.20 Urea at 1.5 M maximal enhancement of activity, at 3 M 50% inhibition Rhizopus arrhizus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 1,10-phenanthroline slight inhibition, 1 mM, 88% remaining activity Rhizopus arrhizus
3.1.1.20 2-mercaptoethanol 1 mM, 68.3% remaining activity Rhizopus arrhizus
3.1.1.20 Ag+ slight competitive inhibition at 1 mM, 82.4% remaining activity Rhizopus arrhizus
3.1.1.20 Ba2+ competitive inhibition at 1 mM, 5.4% remaining activity Rhizopus arrhizus
3.1.1.20 Ca2+ slight competitive inhibition at 1 mM, 86.6% remaining activity Rhizopus arrhizus
3.1.1.20 Co2+ complete inhibition at 1 mM Rhizopus arrhizus
3.1.1.20 CO32-
-
 Rhizopus arrhizus
3.1.1.20 DMSO 1 mM, 19% remaining activity Rhizopus arrhizus
3.1.1.20 EDTA 1 mM, 30% remaining activity Rhizopus arrhizus
3.1.1.20 Fe3+ complete inhibition at 1 mM Rhizopus arrhizus
3.1.1.20 Hg2+ competitive inhibition at 1 mM, 13.8% remaining activity Rhizopus arrhizus
3.1.1.20 OH-
-
 Rhizopus arrhizus
3.1.1.20 S2O82-
-
 Rhizopus arrhizus
3.1.1.20 sodium lauryl sulfate
-
 Rhizopus arrhizus
3.1.1.20 soybean extract inhibits at 0.05-1.0% w/v Rhizopus arrhizus
3.1.1.20 Triton X-100
-
 Rhizopus arrhizus
3.1.1.20 Tween 40 activation up to 0.05% v/v, complete inhibition at 0.6% v/v; activation up to 0.4% v/v, complete inhibition at 0.6% v/v Rhizopus arrhizus
3.1.1.20 Tween 60 complete inhibition at 1% v/v Rhizopus arrhizus
3.1.1.20 Urea at 1.5 M maximal enhancement of activity, at 3 M 50% inhibition Rhizopus arrhizus
3.1.1.20 Zn2+ competitive inhibition at 1 mM, 21.3% remaining activity Rhizopus arrhizus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.20 extracellular
-
 Rhizopus arrhizus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + 10 H2O Rhizopus arrhizus
-
 10 gallic acid + glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Rhizopus arrhizus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + 10 H2O
-
 Rhizopus arrhizus 10 gallic acid + glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Rhizopus arrhizus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 40
-
 assay at Rhizopus arrhizus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.20 0.49
-
 Ba2+ 40°C Rhizopus arrhizus
3.1.1.20 0.5
-
 Ag+ 40°C Rhizopus arrhizus
3.1.1.20 0.57
-
 Hg2+ 40°C Rhizopus arrhizus
3.1.1.20 8.25
-
 Zn2+ 40°C Rhizopus arrhizus