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Literature summary extracted from
- Proline-specific endopeptidases (2003), Russ. J. Bioorg. Chem., 29, 1-17.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.26 | thiol reagents | activate | Oryctolagus cuniculus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.26 | 1,10-phenanthroline | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 3,4-dichloroisocoumarin | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | 5,5'-dithio-bis(2-nitrobenzoic acid) | enzyme from brain | Bos taurus | |
| 3.4.21.26 | alpha-ketobenzothiazol | enzyme from serum | Bos taurus | |
| 3.4.21.26 | bacitracin | - |
Cavia porcellus | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Gly-Pro-CH2Cl | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-CH2Cl | - |
Agaricus bisporus | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-CH2Cl | - |
Ascidia sp. | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-CH2Cl | - |
Bos taurus | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-CH2Cl | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-CH2Cl | enzyme from kidney and brain | Ovis aries | |
| 3.4.21.26 | benzyloxycarbonyl-Pro-prolinal | - |
Agaricus bisporus | |
| 3.4.21.26 | benzyloxycarbonyl-Pro-prolinal | - |
Homo sapiens | |
| 3.4.21.26 | benzyloxycarbonyl-Pro-prolinal | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | benzyloxycarbonyl-Pro-pyrrolidine | - |
Agaricus bisporus | |
| 3.4.21.26 | benzyloxycarbonyl-Pro-thiazolidine | - |
Agaricus bisporus | |
| 3.4.21.26 | benzyloxycarbonyl-thioprolylthiazolidine | - |
Agaricus bisporus | |
| 3.4.21.26 | benzyloxycarbonyl-thioprolylthioprolinal | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | Cd2+ | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | Co2+ | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | Cu2+ | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | Cu2+ | enzyme from brain | Ovis aries | |
| 3.4.21.26 | Cu2+ | enzyme from skin | Rattus norvegicus | |
| 3.4.21.26 | diethyl dicarbonate | enzyme from brain | Sus scrofa | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Agaricus bisporus | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Ascidia sp. | |
| 3.4.21.26 | diisopropyl fluorophosphate | enzyme from hypophysis, eye-lens and brain | Bos taurus | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Clupea pallasii | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | diisopropyl fluorophosphate | enzyme from brain, lymphocyte and serum | Homo sapiens | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Mus musculus | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Novosphingobium capsulatum | |
| 3.4.21.26 | diisopropyl fluorophosphate | enzyme from kidney and brain | Ovis aries | |
| 3.4.21.26 | diisopropyl fluorophosphate | enzyme from brain and skin | Rattus norvegicus | |
| 3.4.21.26 | diisopropyl fluorophosphate | enzyme from brain and muscle | Sus scrofa | |
| 3.4.21.26 | diisopropyl fluorophosphate | - |
Xanthomonas sp. | |
| 3.4.21.26 | E-64 | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | EDTA | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | Hg2+ | enzyme from hypophysis and brain | Bos taurus | |
| 3.4.21.26 | Hg2+ | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | Hg2+ | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | Hg2+ | enzyme from brain | Ovis aries | |
| 3.4.21.26 | iodoacetamide | enzyme from brain | Bos taurus | |
| 3.4.21.26 | iodoacetamide | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | iodoacetamide | enzyme from brain | Rattus norvegicus | |
| 3.4.21.26 | iodoacetate | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | iodoacetate | enzyme from brain | Rattus norvegicus | |
| 3.4.21.26 | Mn2+ | - |
Gallus gallus | |
| 3.4.21.26 | additional information | no effect by SH-reagents and metal chelators | Novosphingobium capsulatum | |
| 3.4.21.26 | NEM | enzyme from hypophysis and eye-lens | Bos taurus | |
| 3.4.21.26 | NEM | enzyme from brain and skin | Rattus norvegicus | |
| 3.4.21.26 | PCMB | - |
Agaricus bisporus | |
| 3.4.21.26 | PCMB | enzyme from brain | Bos taurus | |
| 3.4.21.26 | PCMB | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | PCMB | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | PCMB | enzyme from kidney and brain | Ovis aries | |
| 3.4.21.26 | PCMB | enzyme from brain and skin | Rattus norvegicus | |
| 3.4.21.26 | PCMB | enzyme from brain | Sus scrofa | |
| 3.4.21.26 | PCMBS | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | PMSF | - |
Ascidia sp. | |
| 3.4.21.26 | PMSF | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | PMSF | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | PMSF | enzyme from brain and partly enzyme from skin | Rattus norvegicus | |
| 3.4.21.26 | tosyllysylchloromethane | enzyme from brain | Rattus norvegicus | |
| 3.4.21.26 | tosylphenylalanylchloromethane | enzyme from brain, partly | Rattus norvegicus | |
| 3.4.21.26 | Zn2+ | partly | Gallus gallus | |
| 3.4.21.26 | Zn2+ | enzyme from lymphocyte | Homo sapiens | |
| 3.4.21.26 | Zn2+ | enzyme from skin | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 0.01 | - |
benzyloxycarbonyl-Ala-Pro-p-nitroanilide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.02 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.02 | - |
benzyloxycarbonyl-Gly-Pro-4-methylcoumaryl-7-amide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.025 | - |
benzyloxycarbonyl-Gly-Pro-4-methylcoumaryl-7-amide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.06 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.07 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.07 | - |
benzyloxycarbonyl-Ala-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.08 | - |
benzyloxycarbonyl-Ala-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.08 | - |
Benzyloxycarbonyl-Gly-Pro-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.1 | - |
Oxytocin | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.12 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.125 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.13 | - |
Vasopressin | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.13 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Gly | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.133 | - |
benzyloxycarbonyl-Gly-Gly-Pro-p-nitrophenol | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.14 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.14 | - |
benzyloxycarbonyl-D-Ala-Gly-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.14 | - |
benzyloxycarbonyl-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.14 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-D-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.16 | - |
benzyloxycarbonyl-Ala-Ala-p-nitrophenol | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.16 | - |
Benzyloxycarbonyl-Gly-Pro-Phe | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.17 | - |
Benzyloxycarbonyl-Gly-Pro-Leu | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.18 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.2 | - |
benzyloxycarbonyl-D-Ala-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.22 | - |
Benzyloxycarbonyl-Gly-Pro-Leu | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.25 | - |
benzyloxycarbonyl-Gly-Pro-D-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.27 | - |
benzyloxycarbonyl-Gly-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.29 | - |
benzyloxycarbonyl-Ala-Gly-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.31 | - |
benzyloxycarbonyl-Ala-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.32 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Ala | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.32 | - |
benzyloxycarbonyl-Gly-Pro-D-Leu | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.35 | - |
Ala-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.37 | - |
benzyloxycarbonyl-Gly-Pro-Leu-D-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.38 | - |
benzyloxycarbonyl-D-Ala-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.39 | - |
Benzyloxycarbonyl-Gly-Pro-Ala | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.4 | - |
benzyloxycarbonyl-Gly-Pro-p-nitroanilide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.47 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.58 | - |
benzyloxycarbonyl-Ala-Ala-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.68 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.74 | - |
Benzyloxycarbonyl-Gly-Pro-Phe | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 1.02 | - |
benzyloxycarbonyl-Ala-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 1.4 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Gly | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 1.5 | - |
benzyloxycarbonyl-Gly-Pro-Leu-D-Ala | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 1.67 | - |
benzyloxycarbonyl-D-Ala-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 1.82 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Ala | - |
Elizabethkingia meningoseptica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 66000 | - |
- |
Ascidia sp. |
| 3.4.21.26 | 67000 | 70000 | enzyme from kidney | Sus scrofa |
| 3.4.21.26 | 68000 | - |
- |
Oryctolagus cuniculus |
| 3.4.21.26 | 69700 | - |
enzyme from serum | Bos taurus |
| 3.4.21.26 | 70000 | - |
enzyme from brain | Sus scrofa |
| 3.4.21.26 | 70000 | - |
enzyme from skin | Rattus norvegicus |
| 3.4.21.26 | 70000 | 73000 | enzyme from brain | Rattus norvegicus |
| 3.4.21.26 | 72000 | - |
enzyme from eye-lens | Bos taurus |
| 3.4.21.26 | 72000 | 74000 | enzyme from muscle | Sus scrofa |
| 3.4.21.26 | 74000 | - |
enzyme from serum | Homo sapiens |
| 3.4.21.26 | 74000 | - |
enzyme from kindney | Ovis aries |
| 3.4.21.26 | 75000 | - |
- |
Xanthomonas sp. |
| 3.4.21.26 | 75000 | - |
enzyme from brain | Bos taurus |
| 3.4.21.26 | 76000 | - |
enzyme from hypophysis | Bos taurus |
| 3.4.21.26 | 76000 | - |
1 * 76000, SDS-PAGE | Lyophyllum cinerascens |
| 3.4.21.26 | 77000 | - |
enzyme from brain | Ovis aries |
| 3.4.21.26 | 78000 | - |
1 * 78000, SDS-PAGE | Agaricus bisporus |
| 3.4.21.26 | 78433 | - |
x * 78433, calculation from nucleotide sequence | Novosphingobium capsulatum |
| 3.4.21.26 | 79000 | - |
- |
Mus musculus |
| 3.4.21.26 | 79000 | - |
- |
Clupea pallasii |
| 3.4.21.26 | 79600 | - |
enzyme from brain | Homo sapiens |
| 3.4.21.26 | 80700 | - |
enzyme from lymphocyte | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.26 | Agaricus bisporus | - |
- |
- |
| 3.4.21.26 | Ascidia sp. | - |
- |
- |
| 3.4.21.26 | Bos taurus | - |
- |
- |
| 3.4.21.26 | Cavia porcellus | - |
- |
- |
| 3.4.21.26 | Clupea pallasii | - |
- |
- |
| 3.4.21.26 | Elizabethkingia meningoseptica | - |
- |
- |
| 3.4.21.26 | Gallus gallus | - |
- |
- |
| 3.4.21.26 | Homo sapiens | - |
- |
- |
| 3.4.21.26 | Lactifluus hygrophoroides | - |
- |
- |
| 3.4.21.26 | Lyophyllum cinerascens | - |
- |
- |
| 3.4.21.26 | Mus musculus | - |
- |
- |
| 3.4.21.26 | Novosphingobium capsulatum | - |
IFO 12533 | - |
| 3.4.21.26 | Oryctolagus cuniculus | - |
- |
- |
| 3.4.21.26 | Ovis aries | - |
- |
- |
| 3.4.21.26 | Rattus norvegicus | - |
- |
- |
| 3.4.21.26 | Russula lepida | - |
- |
- |
| 3.4.21.26 | Sus scrofa | - |
- |
- |
| 3.4.21.26 | Xanthomonas sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.26 | - |
Lyophyllum cinerascens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.26 | brain | - |
Homo sapiens | - |
| 3.4.21.26 | brain | - |
Rattus norvegicus | - |
| 3.4.21.26 | brain | - |
Sus scrofa | - |
| 3.4.21.26 | brain | - |
Bos taurus | - |
| 3.4.21.26 | brain | - |
Oryctolagus cuniculus | - |
| 3.4.21.26 | brain | - |
Ovis aries | - |
| 3.4.21.26 | culture filtrate | - |
Agaricus bisporus | - |
| 3.4.21.26 | eye | - |
Bos taurus | - |
| 3.4.21.26 | hypophysis | - |
Bos taurus | - |
| 3.4.21.26 | kidney | - |
Sus scrofa | - |
| 3.4.21.26 | kidney | - |
Bos taurus | - |
| 3.4.21.26 | kidney | - |
Ovis aries | - |
| 3.4.21.26 | lens | - |
Bos taurus | - |
| 3.4.21.26 | liver | - |
Gallus gallus | - |
| 3.4.21.26 | lymphocyte | - |
Homo sapiens | - |
| 3.4.21.26 | muscle | - |
Sus scrofa | - |
| 3.4.21.26 | serum | - |
Homo sapiens | - |
| 3.4.21.26 | serum | - |
Bos taurus | - |
| 3.4.21.26 | skin | - |
Rattus norvegicus | - |
| 3.4.21.26 | skin | granulomatous inflammation | Mus musculus | - |
| 3.4.21.26 | sperm | - |
Ascidia sp. | - |
| 3.4.21.26 | sperm | - |
Clupea pallasii | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.26 | Ala-Gly-Pro-beta-naphthylamide + H2O | - |
Lyophyllum cinerascens | Ala-Gly-Pro + 2-naphthylamine | - |
? | |
| 3.4.21.26 | angiotensin I + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Ala-beta-naphthylamide + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Ala-Ala + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Ala-p-nitrophenol + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Ala-Ala + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Gly-Pro-beta-naphthylamide + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Ala-Gly-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Gly-Pro-beta-naphthylamide + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Ala-Gly-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Gly-Pro-p-nitrophenol + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Ala-Gly-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Pro-beta-naphthylamide + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Ala-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Pro-p-nitroanilide + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Ala-Pro + p-nitroaniline | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Ala-Pro-p-nitrophenol + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Ala-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-D-Ala-Gly-Pro-beta-naphthylamide + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-D-Ala-Gly-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-D-Ala-Gly-Pro-beta-naphthylamide + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-D-Ala-Gly-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-D-Ala-Gly-Pro-p-nitrophenol + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-D-Ala-Gly-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-D-Ala-Pro-beta-naphthylamide + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-D-Ala-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Gly-Pro-p-nitrophenol + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Gly-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Gly-Pro-p-nitrophenol + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Gly-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-4-methylcoumaryl-7-amide + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-4-methylcoumaryl-7-amide + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Ala + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Ala + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Ala + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-beta-naphthylamide + H2O | - |
Agaricus bisporus | benzyloxycarbonyl-Gly-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-beta-naphthylamide + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-beta-naphthylamide + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + beta-naphthylamine | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-D-Ala + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + D-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-D-Leu + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + D-Leu | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Leu | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + Leu | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Leu | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Ala + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Leu-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Ala + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + Leu-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Ala + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Leu-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-D-Ala + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Leu-D-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-D-Ala + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + Leu-D-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-D-Ala + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Leu-D-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Leu-Gly | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + Leu-Gly | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Leu-Gly | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly-Ala + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Leu-Gly-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly-Ala + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Leu-Gly-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly-D-Ala + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Leu-Gly-D-Ala | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly-Gly + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Leu-Gly-Gly | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Leu-Gly-Gly + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Leu-Gly-Gly | - |
? | |
| 3.4.21.26 | Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O | - |
Lyophyllum cinerascens | Benzyloxycarbonyl-Gly-Pro + Leu-Gly-Pro | - |
? | |
| 3.4.21.26 | Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O | enzyme from kidney | Ovis aries | Benzyloxycarbonyl-Gly-Pro + Leu-Gly-Pro | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-p-nitroanilide + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + p-nitroaniline | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-p-nitrophenol + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-p-nitrophenol + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Phe + H2O | - |
Elizabethkingia meningoseptica | benzyloxycarbonyl-Gly-Pro + Phe | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Phe + H2O | - |
Lyophyllum cinerascens | benzyloxycarbonyl-Gly-Pro + Phe | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-Pro-Phe + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Gly-Pro + Phe | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Pro-p-nitrophenol + H2O | enzyme from kidney | Ovis aries | benzyloxycarbonyl-Pro + p-nitrophenol | - |
? | |
| 3.4.21.26 | insulin + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| 3.4.21.26 | additional information | no hydrolysis of benzyloxycarbonyl-D-Pro-p-nitrophenol, negligibly small hydrolysis rate with benzyloxycarbonyl-D-Ala-Pro-p-nitrophenol | Ovis aries | ? | - |
? | |
| 3.4.21.26 | additional information | no hydrolysis of Pro-beta-naphthylamide, benzyloxycarbonyl-Pro-beta-naphthylamide, benzyloxycarbonyl-Ala-p-nitrophenol, Gly-Prp-beta-naphthylamide, Ala-Ala-beta-naphthylamide, benzyloxycarbonyl-Gly-Pro-D-Ala | Elizabethkingia meningoseptica | ? | - |
? | |
| 3.4.21.26 | additional information | no hydrolysis of Pro-beta-naphthylamide, benzyloxycarbonyl-Pro-beta-naphthylamide, Gly-Pro-beta-naphthylamide, benzyloxycarbonyl-Gly-D-Pro-p-nitroanilide, benzyloxycarbonyl-D-Ala-Pro-beta-naphthylamide and benzyloxycarbonyl-Gly-Pro-D-Ala | Lyophyllum cinerascens | ? | - |
? | |
| 3.4.21.26 | additional information | specifically hydrolyzes Pro-Xaa bonds, exerts a low specificity towards residues in position P1', except for Glu and P2 and does not cleave the proline bonds in long substrates such as ribonuclease | Xanthomonas sp. | ? | - |
? | |
| 3.4.21.26 | neurotensin + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| 3.4.21.26 | oxytocin + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| 3.4.21.26 | thyroliberin + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| 3.4.21.26 | tuftsin + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| 3.4.21.26 | Vasopressin + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.26 | ? | x * 78433, calculation from nucleotide sequence | Novosphingobium capsulatum |
| 3.4.21.26 | monomer | 1 * 78000, SDS-PAGE | Agaricus bisporus |
| 3.4.21.26 | monomer | 1 * 76000, SDS-PAGE | Lyophyllum cinerascens |
| 3.4.21.26 | monomer | 1 * 74000-75000 | Elizabethkingia meningoseptica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 40 | - |
- |
Elizabethkingia meningoseptica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 42 | - |
pH 7.0, 15 min, 50% loss of activity | Elizabethkingia meningoseptica |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | additional information | - |
benzyloxycarbonyl-Gly-Pro-Leu-D-Ala | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.031 | 0.51 | Benzyloxycarbonyl-Gly-Pro-Leu-Gly | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.147 | - |
benzyloxycarbonyl-D-Ala-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 0.4 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.67 | - |
benzyloxycarbonyl-Gly-Pro-D-Leu | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 0.82 | - |
benzyloxycarbonyl-Ala-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 0.95 | - |
benzyloxycarbonyl-Gly-Pro-4-methylcoumaryl-7-amide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 1.05 | - |
Benzyloxycarbonyl-Gly-Pro-Leu | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 1.26 | - |
Benzyloxycarbonyl-Gly-Pro-Ala | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 1.52 | - |
benzyloxycarbonyl-Gly-Pro-D-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 2 | 8 | benzyloxycarbonyl-Gly-Pro-Leu-Gly-Gly | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 2 | - |
benzyloxycarbonyl-D-Ala-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 2.05 | - |
benzyloxycarbonyl-Gly-Gly-Pro-p-nitrophenol | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 2.7 | - |
benzyloxycarbonyl-Ala-Ala-p-nitrophenol | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 2.94 | - |
benzyloxycarbonyl-Gly-Pro-D-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 3.2 | - |
Benzyloxycarbonyl-Gly-Pro-Phe | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 3.8 | - |
benzyloxycarbonyl-Gly-Pro-p-nitroanilide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 3.95 | - |
Benzyloxycarbonyl-Gly-Pro-Phe | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 4.2 | - |
benzyloxycarbonyl-Ala-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 5.67 | - |
benzyloxycarbonyl-Gly-Pro-Leu-D-Ala | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 5.9 | - |
benzyloxycarbonyl-Ala-Ala-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 6.08 | - |
Benzyloxycarbonyl-Gly-Pro-Ala | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 6.08 | - |
benzyloxycarbonyl-Gly-Pro-D-Leu | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 7.98 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 8.9 | - |
benzyloxycarbonyl-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 12.4 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 13.2 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Ala | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 15.6 | - |
Benzyloxycarbonyl-Gly-Pro-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 15.9 | - |
Ala-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 23 | - |
Benzyloxycarbonyl-Gly-Pro-Leu | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 25 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-D-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 26 | - |
Benzyloxycarbonyl-Gly-Pro-Leu | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 27 | - |
benzyloxycarbonyl-Gly-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 30 | - |
benzyloxycarbonyl-D-Ala-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 32.7 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 34 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 38 | - |
benzyloxycarbonyl-D-Ala-Gly-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 38.2 | - |
benzyloxycarbonyl-Ala-Gly-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 38.4 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 46 | - |
benzyloxycarbonyl-Gly-Pro-Leu-D-Ala | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 52 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 53.3 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 61 | - |
benzyloxycarbonyl-Ala-Pro-p-nitrophenol | enzyme from kidney | Ovis aries | |
| 3.4.21.26 | 85.8 | - |
benzyloxycarbonyl-Gly-Gly-Pro-p-nitrophenol | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 102 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 115 | - |
benzyloxycarbonyl-Gly-Pro-4-methylcoumaryl-7-amide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 126 | - |
benzyloxycarbonyl-Ala-Pro-p-nitroanilide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 153 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | - |
Lyophyllum cinerascens | |
| 3.4.21.26 | 169 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 180 | - |
Benzyloxycarbonyl-Gly-Pro-Phe | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 192 | - |
benzyloxycarbonyl-Ala-Gly-Pro-beta-naphthylamide | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 240 | - |
Benzyloxycarbonyl-Gly-Pro-Ala | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 520 | - |
Benzyloxycarbonyl-Gly-Pro-Leu-Gly | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 520 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Ala | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 700 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Gly | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 1000 | - |
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Ala | - |
Elizabethkingia meningoseptica | |
| 3.4.21.26 | 1600 | - |
benzyloxycarbonyl-Gly-Pro-Leu-D-Ala | - |
Elizabethkingia meningoseptica | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 5.8 | - |
enzyme from skin | Rattus norvegicus |
| 3.4.21.26 | 6.8 | - |
enzyme from brain | Homo sapiens |
| 3.4.21.26 | 6.8 | - |
hydrolysis of benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | Lyophyllum cinerascens |
| 3.4.21.26 | 7 | 8 | enzyme from brain | Rattus norvegicus |
| 3.4.21.26 | 7 | 8 | enzyme from lymphocyte | Homo sapiens |
| 3.4.21.26 | 7 | - |
- |
Ascidia sp. |
| 3.4.21.26 | 7 | - |
enzyme from brain | Ovis aries |
| 3.4.21.26 | 7 | - |
reaction witrh benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | Elizabethkingia meningoseptica |
| 3.4.21.26 | 7.2 | 7.5 | enzyme from brain | Bos taurus |
| 3.4.21.26 | 7.4 | 7.6 | enzyme from hypophysis | Bos taurus |
| 3.4.21.26 | 7.5 | - |
- |
Gallus gallus |
| 3.4.21.26 | 7.5 | - |
- |
Oryctolagus cuniculus |
| 3.4.21.26 | 7.5 | - |
- |
Xanthomonas sp. |
| 3.4.21.26 | 7.5 | - |
enzyme from serum | Homo sapiens |
| 3.4.21.26 | 7.5 | - |
enzyme from eye-lens | Bos taurus |
| 3.4.21.26 | 7.5 | - |
hydrolysis of benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | Agaricus bisporus |
| 3.4.21.26 | 7.8 | - |
enzyme from kindney | Ovis aries |
| 3.4.21.26 | 8 | 8.5 | enzyme from serum | Bos taurus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.26 | Rattus norvegicus | enzyme from brain | - |
4.5 |
| 3.4.21.26 | Agaricus bisporus | - |
- |
4.8 |
| 3.4.21.26 | Bos taurus | enzyme from brain | - |
4.8 |
| 3.4.21.26 | Homo sapiens | enzyme from brain and lymphocyte | - |
4.8 |
| 3.4.21.26 | Ovis aries | enzyme from kindney | - |
4.8 |
| 3.4.21.26 | Oryctolagus cuniculus | - |
- |
4.9 |
| 3.4.21.26 | Sus scrofa | enzyme from brain | - |
4.9 |
| 3.4.21.26 | Ovis aries | enzyme from brain | - |
4.9 |
| 3.4.21.26 | Lyophyllum cinerascens | - |
- |
5.2 |
| 3.4.21.26 | Ascidia sp. | - |
- |
5.5 |
| 3.4.21.26 | Xanthomonas sp. | - |
- |
6.2 |
| 3.4.21.26 | Elizabethkingia meningoseptica | - |
- |
9.6 |
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