Literature summary extracted from

Nastopoulos, V.; Vallone, B.; Politi, L.; Scotto d'Abusco, A.; Scandurra, R.; Tsernoglou, D.
Crystallization and X-ray diffraction measurements of a thermophilic archaeal recombinant amidase from Sulfolobus solfataricus MT4 (2001), Acta Crystallogr. Sect. D, D57, 1036-1037.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.1.4	expression in Escherichia coli of a recombinant His-tagged protein	Saccharolobus solfataricus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.5.1.4	native enzyme using the hanging-drop vapour-diffudion technique, a His-tagged protein cannot be crystallized	Saccharolobus solfataricus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.4	Saccharolobus solfataricus	-	MT4 strain	-
3.5.1.4	Saccharolobus solfataricus MT4	-	MT4 strain	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.4	extraction of a His-tagged enzyme from insoluble cellular fractions using sodium phosphate buffer at pH 7.8 and further purification by affinity column chromatography	Saccharolobus solfataricus

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Release 2023.1 (January 2023)