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Literature summary extracted from

  • Overhage, J.; Steinbuchel, A.; Priefert, H.
    Biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16 (2002), Appl. Environ. Microbiol., 68, 4315-4321.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.90 biotechnology biotechnological production of vanillin Pseudomonas putida
1.1.1.90 synthesis biotechnological production of vanillin Cupriavidus necator
1.2.1.68 biotechnology biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. The gene calB, encoding coniferyl aldehyde dehydrogenase, and ehyAB and calA encoding eugenol hydroxylase and coniferyl alcohol dehydrogenase, respectively, are amplified and combined to construct a catabolic gene cassette. This gene cassette is cloned in the broad-host-range vector pBBR1-JO2 and transferred to Ralstonia eutropha H16. A recombinant strain of Ralstonia eutropha H16 harboring this plasmid expresses functionally active eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase. Cells of Ralstonia eutropha H16 from the late-exponential growth phase are used asa biocatalysts for the biotransformation of eugenol to ferulic acid. A maximum conversion rate of 2.9 mM of eugenol per h per liter of culture is achieved with a yield of 93.8 mol% of ferulic acid from eugenol within 20 h, without further optimization Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.90 expression in Escherichia coli XL1-Blue Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.68 coniferyl aldehyde + H2O + NAD+ Pseudomonas sp. involved in eugenol catabolism ferulate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Cupriavidus necator
-
 recombinant strain H16(pBBR1-JO2ehyABcalAcalB)
-
1.1.1.90 Pseudomonas putida
-
-
-
1.2.1.68 Pseudomonas sp. Q86447
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.90 0.22
-
 recombinant strain Cupriavidus necator
1.1.1.90 0.24
-
 comparison to recombinant enzymes Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 coniferyl alcohol + NAD+ biotransformation of eugenol to ferulic acid Pseudomonas putida coniferyl aldehyde + NADH + H+
-
 ?
1.1.1.90 coniferyl alcohol + NAD+ biotransformation of eugenol to ferulic acid Cupriavidus necator coniferyl aldehyde + NADH + H+
-
 ?
1.2.1.68 coniferyl aldehyde + H2O + NAD+
-
 Pseudomonas sp. ferulate + NADH
-
 ?
1.2.1.68 coniferyl aldehyde + H2O + NAD+ involved in eugenol catabolism Pseudomonas sp. ferulate + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.68 coniferyl aldehyde dehydrogenase
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Pseudomonas putida
1.1.1.90 NAD+
-
 Cupriavidus necator