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Literature summary extracted from

  • Wu, C.; Colleoni, C.; Myers, A.M.; James, M.G.
    Enzymatic properties and regulation of ZPU1, the maize pullulanase-type starch debranching enzyme (2002), Arch. Biochem. Biophys., 406, 21-32.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.41 dithiothreitol upon preincubation with both dithiothreitol and thioredoxin, the initial reaction velocity increases by a factor of two Zea mays
3.2.1.41 thioredoxin upon preincubation with both dithiothreitol and thioredoxin, the initial reaction velocity increases by a factor of two Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 0.37
-
 pullulan pH 6.0, 37°C Zea mays
3.2.1.41 4.1
-
 beta-limit dextrin pH 6.0, 37°C Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Zea mays O81638
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 recombinant protein expressed in Escherichia coli Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 15.5
-
 substrate phytoglycogen, pH 6.0, 37°C Zea mays
3.2.1.41 32.7
-
 substrate amylopectin, pH 6.0, 37°C Zea mays
3.2.1.41 165.9
-
 substrate pullulan, pH 6.0, 37°C Zea mays
3.2.1.41 223.9
-
 substrate beta-limit dextrin, pH 6.0, 37°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O 20% of activity compared to pullulan Zea mays ?
-
 ?
3.2.1.41 beta-limit dextrin + H2O
-
 Zea mays ?
-
 ?
3.2.1.41 beta-limit dextrin + H2O 135% of activity compared to pullulan Zea mays ?
-
 ?
3.2.1.41 additional information enzyme requires at least two glucose units in linear chain to be released by cleavage of an alpha-1,6-linkage Zea mays ?
-
 ?
3.2.1.41 additional information no substrate: phytogen Zea mays ?
-
 ?
3.2.1.41 phytoglycogen + H2O 9% of activity compared to pullulan Zea mays ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Zea mays ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Zea mays maltotriose + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 42
-
 stable Zea mays
3.2.1.41 50
-
 complete inactivation within 15 min Zea mays