EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.40 | expression of wild-type and mutants in Escherichia coli BL21(DE3) | Zea mays |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.40 | K225I | site-directed mutagenesis, mutation of a conserved residue involved in catalysis and substrate binding, mutant shows highly reduced activity and a 10fold higher partitioning ratio of oxaloacetate and malate compared to the wild-type enzyme, preference for reduction of oxaloacetate instead of decarboxylation | Zea mays |
1.1.1.40 | K435L/K436L | site-directed mutagenesis, mutation of residues which are important in cofactor binding, over 6fold increased Ki for 2'-AMP, and 1.7fold decreased Ki for 5'-AMP, and increased activity with NAD+ compared to the wild-type enzyme | Zea mays |
1.1.1.40 | R237L | site-directed mutagenesis, mutation of a conserved residue involved in catalysis and substrate binding, mutant shows and an over 100fold higher partitioning ratio of oxaloacetate and malate compared to the wild-type enzyme, preference for reduction of oxaloacetate instead of decarboxylation | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | 2'-AMP | competitive | Zea mays | |
1.1.1.40 | 5'-AMP | competitive | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.008 | - |
NADP+ | wild-type enzyme, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.073 | - |
NADP+ | mutant K435L/K436L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.123 | - |
NADP+ | mutant K225I, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.23 | - |
L-malate | wild-type enzyme, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.29 | - |
NADP+ | mutant R237L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.31 | - |
L-malate | mutant K435L/K436L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 1.9 | - |
NAD+ | mutant K435L/K436L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 2.6 | - |
L-malate | mutant K225I, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 2.9 | - |
L-malate | mutant R237L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 8.1 | - |
NAD+ | wild-type enzyme, pH 8.0, 30°C | Zea mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | Mg2+ | - |
Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.40 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.40 | recombinant wild-type and mutants from Escherichia coli to homogeneity | Zea mays |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.40 | (S)-malate + NADP+ = pyruvate + CO2 + NADPH + H+ | the conserved residues Arg237 and Lys225 are involved in catalysis and substrate binding as proton acceptors | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.40 | (S)-malate + NAD+ | - |
Zea mays | pyruvate + CO2 + NADH | - |
? | |
1.1.1.40 | (S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH | - |
? | |
1.1.1.40 | Oxaloacetate | - |
Zea mays | Pyruvate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.40 | NADP+-dependent malic enzyme | - |
Zea mays |
1.1.1.40 | NADP+-ME | - |
Zea mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 30 | - |
assay at | Zea mays |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.38 | - |
NADP+ | mutant R237L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 1.1 | - |
NADP+ | mutant K225I, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 13.5 | - |
NAD+ | wild-type enzyme, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 18.4 | - |
NAD+ | mutant K435L/K436L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 181.1 | - |
NADP+ | mutant K435L/K436L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 201.3 | - |
NADP+ | wild-type enzyme, pH 8.0, 30°C | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 8 | - |
assay at | Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | NAD+ | very low activity with | Zea mays | |
1.1.1.40 | NADP+ | cofactor specificity is determined by Lys435/Lys436 interacting with the 2'-phosphate group of the ribose ring | Zea mays |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.105 | - |
2'-AMP | wild-type enzyme, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.256 | - |
5'-AMP | mutant K435L/K436L, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.439 | - |
5'-AMP | wild-type enzyme, pH 8.0, 30°C | Zea mays | |
1.1.1.40 | 0.688 | - |
2'-AMP | mutant K435L/K436L, pH 8.0, 30°C | Zea mays |