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Literature summary extracted from

  • Seah, S.Y.; Britton, K.L.; Rice, D.W.; Asano, Y.; Engel, P.C.
    Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates (2002), Biochemistry, 41, 11390-11397.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.20 expression in Escherichia coli Lysinibacillus sphaericus

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.20 N145A 50% of wild-type kcat with phenylalanine Lysinibacillus sphaericus
1.4.1.20 N145I 19% of wild-type kcat with phenylalanine Lysinibacillus sphaericus
1.4.1.20 N145L 12.5% of wild-type kcat with phenylalanine Lysinibacillus sphaericus
1.4.1.20 N145V 25% of wild-type kcat with phenylalanine Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 0.087
-
 L-phenylalanine
-
 Bacillus badius
1.4.1.20 0.14
-
 L-isoleucine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 0.19
-
 p-hydroxy-phenylpyruvate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 0.37
-
 phenylpyruvate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 0.55
-
 L-leucine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 0.79
-
 L-norleucine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 1.1
-
 L-phenylalanine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 1.3
-
 L-tyrosine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 2
-
 L-valine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 2.7
-
 L-methionine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 3.3
-
 L-norvaline 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 8.6
-
 L-tyrosine
-
 Bacillus badius
1.4.1.20 11
-
 alpha-keto-gamma-methiobutyrate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 13
-
 alpha-Ketocaproate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 13
-
 DL-alpha-keto-beta-methyl-n-valerate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 20
-
 4-methyl-2-oxopentanoate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 52
-
 alpha-ketovalerate 25°C, pH 10.4 Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 39000
-
 10 * 39000, SDS-PAGE Lysinibacillus sphaericus
1.4.1.20 380000
-
 gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.20 L-phenylalanine + H2O + NAD+ Lysinibacillus sphaericus
-
 phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+ Bacillus badius
-
 phenylpyruvate + NH3 + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Bacillus badius
-
-
-
1.4.1.20 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20 recombinant PheDH Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 Lysinibacillus sphaericus L-leucine + NAD+ + H2O
-
 r
1.4.1.20 L-isoleucine + H2O + NAD+
-
 Lysinibacillus sphaericus 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.20 L-leucine + H2O + NAD+
-
 Lysinibacillus sphaericus 3,3-dimethyl-2-oxo-butanoate + NH3 + NADH
-
 r
1.4.1.20 L-methionine + H2O + NAD+
-
 Lysinibacillus sphaericus (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH
-
 r
1.4.1.20 L-norleucine + H2O + NAD+
-
 Lysinibacillus sphaericus 2-oxohexanoate + NH3 + NADH
-
 r
1.4.1.20 L-norvaline + H2O + NAD+
-
 Lysinibacillus sphaericus 2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Bacillus badius phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-tyrosine + H2O + NAD+
-
 Lysinibacillus sphaericus (4-hydroxyphenyl)pyruvate + NH3 + NADH
-
 r
1.4.1.20 L-tyrosine + H2O + NAD+
-
 Bacillus badius (4-hydroxyphenyl)pyruvate + NH3 + NADH
-
 r
1.4.1.20 L-valine + H2O + NAD+
-
 Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.20 decamer 10 * 39000, SDS-PAGE Lysinibacillus sphaericus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.20 PheDH
-
 Lysinibacillus sphaericus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.20 0.14
-
 L-isoleucine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 0.7
-
 L-leucine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 0.91
-
 L-valine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 1.2
-
 L-methionine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 1.4
-
 L-norvaline 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 3.2
-
 L-norleucine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 7.2
-
 DL-alpha-keto-beta-methyl-n-valerate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 27
-
 4-methyl-2-oxopentanoate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 27
-
 alpha-keto-gamma-methiobutyrate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 33
-
 alpha-ketovalerate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 34
-
 L-tyrosine
-
 Bacillus badius
1.4.1.20 39
-
 L-phenylalanine
-
 Bacillus badius
1.4.1.20 51
-
 alpha-Ketocaproate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 68
-
 L-tyrosine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 68.01
-
 L-phenylalanine 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 90
-
 p-hydroxy-phenylpyruvate 25°C, pH 10.4 Lysinibacillus sphaericus
1.4.1.20 113
-
 phenylpyruvate 25°C, pH 10.4 Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NAD+
-
 Lysinibacillus sphaericus
1.4.1.20 NAD+
-
 Bacillus badius
1.4.1.20 NADH
-
 Lysinibacillus sphaericus